Back to Search
Start Over
IFN-β modulates the response to TLR stimulation in human DC: Involvement of IFN regulatory factor-1 (IRF-1) in IL-27 gene expression
- Source :
- European Journal of Immunology. 37:3499-3508
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Type I IFN are cytokines which play a central role in host resistance to viral or microbial infections and are important components linking innate and adaptive immunity. We and others have previously demonstrated that the production of IFN-beta by DC following bacterial infections or TLR triggering influences, in an autocrine manner, their maturation. In this study, we investigated whether IFN-beta release modulates the phenotype of the immature DC and their response to a subsequent TLR stimulation. The induction of CD86, HLA-DR, CD38 and B7H1 and the absence of CCR7 and CD83 expression upon IFN-beta treatment suggest that IFN-beta-primed DC remain at the site of infection acquiring an activated phenotype. These results prompted us to investigate the response of IFN-beta-primed DC to TLR stimulation. While IFN-beta pretreatment increases slightly the expression of maturation markers in TLR2- or TLR4-stimulated DC, it is able to modulate selectively the secretion of inflammatory and immuno-regulating cytokines. Interestingly, IL-27p28 subunit was induced by IFN-beta alone or during LPS-induced maturation of DC in a type I IFN-dependent manner through IFN regulatory factor-1 (IRF-1) activation. Taken together, our results shed light on the capacity of IFN-beta to finely tune DC response to invading pathogens.
- Subjects :
- Lipopolysaccharides
Receptors, CCR7
Immunology
Immunoglobulins
Stimulation
C-C chemokine receptor type 7
Biology
Antigens, CD
Humans
Immunology and Allergy
Secretion
Autocrine signalling
Cells, Cultured
CD86
Membrane Glycoproteins
Innate immune system
Interleukin-6
Tumor Necrosis Factor-alpha
Interleukins
Dendritic Cells
Interferon-beta
Acquired immune system
Interleukin-12
Toll-Like Receptor 2
Interleukin-10
Toll-Like Receptor 4
Protein Subunits
TLR2
Gene Expression Regulation
B7-2 Antigen
Interferon Regulatory Factor-1
Signal Transduction
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....dad2caf916534b194ee13ec10cec44fa