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Amniotic fluid proteomic signatures of cervical insufficiency and their association with length of latency
- Source :
- American Journal of Reproductive Immunology. 80:e13030
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Cervical insufficiency is a precursor of preterm birth. Treatment with emergency cervical cerclage is contraindicated in the presence of intra-amniotic infection. Detecting infection with Gram stain and culture of amniotic fluid lacks sensitivity. Proteomic profiling of amniotic fluid in cervical insufficiency may help identify pregnancies best suited for emergency cerclage.Thirty-two pregnant women underwent amniocentesis for routine genetic testing (n = 22) or after diagnosis of cervical insufficiency (n = 10). The proteomic profiles of the amniotic fluid samples were compared in a cross-sectional fashion, including sub-analyses of women with cervical insufficiency and latency periods of1 week and1 week post-diagnosis.Mean gestational age at diagnosis of cervical insufficiency was 21.4 weeks (95% CI 20.6-22.1). Proteomic analysis yielded 40 (7.2%, P 0.05) differentially expressed proteins between women with delivery1 week (n = 6) vs.1 week (n = 4). Women who delivered1 week had activated inflammatory response (z = 2.3, P = 6.71E-09), chemotaxis of immune cells (z = 2.9, P = 2.01E-08), and inhibited bacterial growth (z = -2.2, P = 5.82E-05). A multivariate model of eight biomarkers positively associated with cases of1 week latency and distinguished cases from controls (97.8%, cross-validation accuracy 92.7%, P = 0.0009).In this pilot study, significant differences in the amniotic fluid proteomic profiles in cases of cervical insufficiency compared to genetic amniocentesis were observed. Proteomic signatures were predictive of achieving latency1 week after diagnosis of cervical insufficiency. These preliminary findings suggest that proteomic analysis may be of value in predicting outcome following cervical insufficiency and warrants further validation in larger studies.
- Subjects :
- Adult
0301 basic medicine
medicine.medical_specialty
Amniotic fluid
Cervical insufficiency
Proteome
medicine.medical_treatment
Immunology
Gestational Age
Pilot Projects
Cervix Uteri
Chorioamnionitis
Young Adult
03 medical and health sciences
0302 clinical medicine
Predictive Value of Tests
medicine
Humans
Immunology and Allergy
Cervical cerclage
Retrospective Studies
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
business.industry
Proteomic Profiling
Obstetrics
Obstetrics and Gynecology
Gestational age
Amniotic Fluid
Prognosis
medicine.disease
3. Good health
Cross-Sectional Studies
030104 developmental biology
Reproductive Medicine
Premature birth
Amniocentesis
Premature Birth
Female
Inflammation Mediators
Transcriptome
business
Subjects
Details
- ISSN :
- 10467408
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- American Journal of Reproductive Immunology
- Accession number :
- edsair.doi.dedup.....dacf66dd984663afb43a6f67d8964170