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The synthetic potential of fungal feruloyl esterases
- Source :
- Catalysts, 8(6). Multidisciplinary Digital Publishing Institute, Catalysts (2073-4344) vol.8(2018), Catalysts, Vol 8, Iss 6, p 242 (2018), Antonopoulou, I, Dilokpimol, A, Iancu, L, Mäkelä, M R, Varriale, S, Cerullo, G, Hüttner, S, Uthoff, S, Jütten, P, Piechot, A, Steinbüchel, A, Olsson, L, Faraco, V, Hildén, K S, de Vries, R P, Rova, U & Christakopoulos, P 2018, ' The synthetic potential of fungal feruloyl esterases : A correlation with current classification systems and predicted structural properties ', Catalysts, vol. 8, no. 6, 242 . https://doi.org/10.3390/catal8060242, Catalysts, Volume 8, Issue 6, Catalysts, 8(6). Multidisciplinary Digital Publishing Institute (MDPI)
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.
- Subjects :
- 0106 biological sciences
0301 basic medicine
sugar esters
antioxidant
ENZYMATIC-SYNTHESIS
116 Chemical sciences
Glyceryl ferulate
Alcohol
l-arabinose+ferulate%22">l-arabinose ferulate
lcsh:Chemical technology
01 natural sciences
Antioxidants
lcsh:Chemistry
chemistry.chemical_compound
Feruloyl esterase
CRYSTAL-STRUCTURE
lcsh:TP1-1185
L-arabinose ferulate
Prenyl caffeate
1183 Plant biology, microbiology, virology
chemistry.chemical_classification
Butyl ferulate
biology
Prenyl ferulate
Sugar esters
FAMILY
OLIGOSACCHARIDES
Biocatalysis and Enzyme Technology
antioxidants
prenyl ferulate
ESTERIFICATION
Stereochemistry
Phylogenetic classification
butyl ferulate
ASPERGILLUS-NIGER
FUSARIUM-OXYSPORUM
Catalysis
03 medical and health sciences
l-arabinose+ferulate%22">">l-arabinose ferulate
Prenylation
010608 biotechnology
phylogenetic classification
feruloyl esterase
Homology modeling
Physical and Theoretical Chemistry
OPTIMIZATION
Bioprocess Technology
glyceryl ferulate
prenyl caffeate
l-arabinose ferulate
Aspergillus niger
Organic Chemistry
Transesterification
biology.organism_classification
Xylan
transesterification
030104 developmental biology
Enzyme
chemistry
lcsh:QD1-999
ACID ESTERASES
CATALYZED SYNTHESIS
Subjects
Details
- Language :
- English
- ISSN :
- 20734344
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Catalysts
- Accession number :
- edsair.doi.dedup.....dacd468287731cdb4ad04a8ef68460a7