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Modifications of the nucleoprotein of viral haemorrhagic septicaemia virus showed gain of virulence in intraperitoneally infected rainbow trout
- Source :
- Journal of Fish Diseases, Journal of Fish Diseases, Wiley, 2021, 44 (9), pp.1369-1383. ⟨10.1111/jfd.13395⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Viral haemorrhagic septicaemia virus (VHSV) is the cause of an important listed disease in European rainbow trout (Oncorhynchus mykiss) aquaculture and can be present in a wide range of fish species, including marine fish, which can act as viral reservoir. Recent studies revealed putative genetic virulence markers of VHSV to rainbow trout highlighting the roles of the nucleoprotein, phosphoprotein and non-virion protein. Using reverse genetics, we produced recombinant viruses by introducing parts of or the entire nucleoprotein from a high-virulent isolate VHSV into a low-virulent backbone. Furthermore, we also made recombinant viruses by introducing residue modifications in the nucleoprotein that seem to play a role in virulence. Rainbow trout challenged with these recombinant viruses (rVHSVs) by intraperitoneal injection (IP) developed clinical signs and showed lower survival when compared to the parental rVHSV whereas fish challenged by immersion did not show clinical signs except for the high-virulent control. The mutations did not influence the viral growth in cell culture. The recombinant viruses and parental recombinant were unable to replicate and show cytopathic effect in EPC cells whereas the high-virulent control was well adapted in all the fish cell lines tested. We showed evidence that corroborates with the hypothesis that the nucleoprotein has virulence motifs associated with VHSV virulence in rainbow trout.
- Subjects :
- 0301 basic medicine
endocrine system
animal structures
Veterinary (miscellaneous)
animal diseases
viruses
Virulence
Aquatic Science
Biology
law.invention
Cell Line
Novirhabdovirus
03 medical and health sciences
Fish Diseases
reverse genetics
law
viral haemorrhagic septicaemia virus
Hemorrhagic Septicemia, Viral
Animals
14. Life underwater
Cytopathic effect
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
urogenital system
Fishes
virulence markers
04 agricultural and veterinary sciences
Virology
rainbow trout
Reverse genetics
Nucleoprotein
030104 developmental biology
Nucleoproteins
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Cell culture
Phosphoprotein
Oncorhynchus mykiss
040102 fisheries
Recombinant DNA
0401 agriculture, forestry, and fisheries
Rainbow trout
Injections, Intraperitoneal
Subjects
Details
- Language :
- English
- ISSN :
- 01407775 and 13652761
- Database :
- OpenAIRE
- Journal :
- Journal of Fish Diseases, Journal of Fish Diseases, Wiley, 2021, 44 (9), pp.1369-1383. ⟨10.1111/jfd.13395⟩
- Accession number :
- edsair.doi.dedup.....dabb5fb132a26952053956965fab9afb