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Ruegeria denitrificans sp. nov., a marine bacterium in the family Rhodobacteraceae with the potential ability for cyanophycin synthesis
- Source :
- International journal of systematic and evolutionary microbiology. 68(8)
- Publication Year :
- 2018
-
Abstract
- Strain CECT 5091T, an aerobic, marine, Gram-reaction- and Gram-stain-negative, chemoheterotrophic bacterium was isolated from oysters harvested off the Spanish Mediterranean coast. Analysis of the 16S rRNA gene sequence placed the strain within the genus Ruegeria , in the family Rhodobacteraceae , with 16S rRNA gene similarities of 98.7, 98.7 and 98.4 % to Ruegeria conchae , Ruegeria atlantica and Ruegeria arenilitoris , respectively. Average nucleotide identities (ANI) and in silico DNA–DNA hybridization (DDH) were determined, comparing the genome sequence of CECT 5091T with those of the type strains of 12 species of the genus Ruegeria : the values obtained were always below the thresholds (95–96 % ANI, 70 % in silico DDH) used to define genomic species, proving that CECT 5091T represents a novel species of the genus Ruegeria . The strain was slightly halophilic and mesophilic, with optimum growth at 26 °C, pH 7.0 and 3 % salinity, it required sodium and magnesium ions for growth and was able to reduce nitrate to dinitrogen. Carbon sources for growth include some carbohydrates (d-ribose, d-glucose, l-rhamnose, N-acetyl-d-glucosamine) and multiple organic acids and amino acids. The major cellular fatty acid was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), representing 70 % of the total fatty acids. Carbon monoxide oxidation, cyanophycin synthetic ability and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine production are predicted from genome annotation, while bacteriochlorophyll a production was absent. The DNA G+C content of the genome was 56.7 mol%. We propose the name Ruegeria denitrificans sp. nov. and strain CECT 5091T (=5OM10T=LMG 29896T) as the type strain for the novel species.
- Subjects :
- 0301 basic medicine
DNA, Bacterial
Cyanophycin
Ruegeria
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Marine bacteriophage
Bacterial Proteins
RNA, Ribosomal, 16S
Mediterranean Sea
Animals
Rhodobacteraceae
Magnesium ion
Ecology, Evolution, Behavior and Systematics
Phylogeny
Base Composition
biology
Fatty Acids
Nucleic Acid Hybridization
General Medicine
Sequence Analysis, DNA
16S ribosomal RNA
biology.organism_classification
Ostreidae
Halophile
Bacterial Typing Techniques
030104 developmental biology
Biochemistry
chemistry
Spain
Bacteria
Subjects
Details
- ISSN :
- 14665034
- Volume :
- 68
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International journal of systematic and evolutionary microbiology
- Accession number :
- edsair.doi.dedup.....dab678a889a8dc8d79a8039d23439c64