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Phenotype microarray analysis of the drug efflux systems in Salmonella enterica serovar Typhimurium
- Source :
- Journal of Infection and Chemotherapy. (11):780-784
- Publisher :
- The Authors. Published by Elsevier Ltd on behalf of Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases.
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Abstract
- A large number of drug efflux transporters have been identified in Salmonella enterica serovar Typhimurium, and increased expression of these transporters confers drug resistance in this organism. Here we compared the respiration activities of the wild-type strain and a mutant with nine deleted transporters by phenotype microarray analysis. The mutant was susceptible to 66 structurally unrelated compounds including many antibiotics, dyes, detergents, antihistamine agents, plant alkaloids, antidepressants, antipsychotic drugs, and antiprotozoal drugs. To investigate the effect of each transporter on the susceptibilities to these drugs, we used the single transporter mutants, several multiple deletion mutants, and the transporter overexpressor strains to determine minimum inhibitory concentrations of ampicillin, erythromycin, minocycline, ciprofloxacin, orphenadrine, amitriptyline, thioridazine, and chlorpromazine. The data indicate that the increased susceptibilities of the mutant lacking nine transporter genes are mainly dependent on the absence of the acrAB efflux genes as well as the tolC gene. In addition to the AcrAB-TolC efflux system, the results from the overexpressor strains show that AcrEF confers resistance to these compounds as well as AcrAB of Escherichia coli, MexAB-OprM and MexXY-OprM of Pseudomonas aeruginosa. The results highlight the importance of the efflux systems not only for resistance to antibiotics but also for resistance to antihistamine agents, plant alkaloids, antidepressants, antipsychotic drugs, and antiprotozoal drugs.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Salmonella typhimurium
S. enterica
030106 microbiology
Mutant
Drug resistance
Microbial Sensitivity Tests
Biology
Pharmacology
Multidrug resistance
medicine.disease_cause
Serogroup
Microbiology
03 medical and health sciences
Bacterial Proteins
Drug Resistance, Multiple, Bacterial
medicine
Escherichia coli
Multidrug efflux pump
Pharmacology (medical)
Bacteria
Pseudomonas aeruginosa
Phenotype microarray
Membrane Transport Proteins
Transporter
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Microarray Analysis
Anti-Bacterial Agents
Multiple drug resistance
030104 developmental biology
Infectious Diseases
Phenotype
Salmonella enterica
Mutation
Efflux
Subjects
Details
- Language :
- English
- ISSN :
- 1341321X
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Infection and Chemotherapy
- Accession number :
- edsair.doi.dedup.....dab39c89cc705262099e8fb530a4e4dd
- Full Text :
- https://doi.org/10.1016/j.jiac.2016.03.015