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Quantitative Analysis with Atomic Force Microscopy of Cisplatin-induced Morphological Changes in HeLa and Ishikawa Cells
- Source :
- Journal of Nippon Medical School. 79:320-326
- Publication Year :
- 2012
- Publisher :
- Medical Association of Nippon Medical School, 2012.
-
Abstract
- Because the cell membrane is an important regulator of cell function, its morphological changes are important markers of cell apoptosis. These changes can differ for each cell type, and depend on the treatment conditions, including the drug, doses, and treatment time. To quantify morphological changes, HeLa and Ishikawa cells were investigated with atomic force microscopy. Both cells were treated with cisplatin (1 mM) for 24 hours. The viability and proliferation of the cells were analyzed with methylthiazol tetrazolium method. The proliferation rates of both cells treated with cisplatin decreased more than 50%. The morphological changes induced by cisplatin were dependent on the cell type, and the results were determined quantitatively. The surface of HeLa cells became rougher with cisplatin treatment, whereas cisplatin-treated Ishikawa cells were smoother than untreated cells. In both cases, cell height was decreased with cisplatin treatment. These results suggest that atomic force microscopy can be used to analyze anticancer drug activity in cancer cells.
- Subjects :
- Cell type
Cell Survival
Cell
Antineoplastic Agents
Microscopy, Atomic Force
HeLa
Cell membrane
Tumor Cells, Cultured
medicine
Humans
Cisplatin
biology
Chemistry
General Medicine
biology.organism_classification
Molecular biology
Endometrial Neoplasms
medicine.anatomical_structure
Apoptosis
Cancer cell
Female
Carcinoma, Endometrioid
Quantitative analysis (chemistry)
HeLa Cells
medicine.drug
Subjects
Details
- ISSN :
- 13473409 and 13454676
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Journal of Nippon Medical School
- Accession number :
- edsair.doi.dedup.....da8cbcaa01f1f5766df80fe367a4ba0d