Back to Search
Start Over
Droplet Digital PCR Is a Robust Tool for Monitoring Minimal Residual Disease in Adult Philadelphia-Positive Acute Lymphoblastic Leukemia
- Source :
- The Journal of Molecular Diagnostics. 20:474-482
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The breakpoint cluster region-abelson 1 p190 fusion transcript is the most frequent variant observed in Philadelphia-positive (Ph+) acute lymphoblastic leukemia (ALL). Qualitative-PCR and real-time quantitative PCR are the currently used methods to monitor minimal residual disease (MRD) in Ph+ ALL patients; for the latter, full standardization and an international quality validation are lacking. Here, we developed a droplet digital PCR (ddPCR) assay for MRD monitoring in p190+ ALL cases. The analytical performance was assessed by the limit-of-detection determination, showing a reliability, sensitivity, and precision of the assay of up to 0.001%. Comparison of results obtained with qualitative PCR and ddPCR in 117 follow-up samples from 16 of 26 Ph+ ALL patients showed discordant results in 27% of cases (32 of 117). Real-time quantitative PCR analysis of 19 ddPCR-positive samples with a low tumor burden failed to provide quantitative results in 63% of cases (12 of 19). These results highlight that in p190+ ALL the ddPCR method has a sufficient analytical performance for very low MRD monitoring and for predicting molecular relapse several months before hematologic relapse. In conclusion, MRD monitoring by ddPCR may better stratify Ph+ ALL patients at risk of disease progression.
- Subjects :
- Adult
Male
0301 basic medicine
Oncology
Acute promyelocytic leukemia
medicine.medical_specialty
Neoplasm, Residual
Fusion Proteins, bcr-abl
Real-Time Polymerase Chain Reaction
Philadelphia chromosome
Pathology and Forensic Medicine
law.invention
Young Adult
03 medical and health sciences
0302 clinical medicine
Limit of Detection
law
hemic and lymphatic diseases
Internal medicine
medicine
Humans
Philadelphia Chromosome
Digital polymerase chain reaction
Polymerase chain reaction
Aged
business.industry
Imatinib
Middle Aged
Precursor Cell Lymphoblastic Leukemia-Lymphoma
medicine.disease
Minimal residual disease
030104 developmental biology
Real-time polymerase chain reaction
Fusion transcript
030220 oncology & carcinogenesis
Molecular Medicine
Female
business
medicine.drug
Subjects
Details
- ISSN :
- 15251578
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The Journal of Molecular Diagnostics
- Accession number :
- edsair.doi.dedup.....da88d6fd5258f6e3b486fb0c914a8675