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14-3-3 zeta protein secreted by tumor associated monocytes/macrophages from ascites of epithelial ovarian cancer patients
- Source :
- Cancer immunology, immunotherapy : CII. 58(2)
- Publication Year :
- 2008
-
Abstract
- Tumor associated monocytes/macrophages (MO/MA) are known contributors to the immune-inflammatory cell environment of advanced epithelial ovarian carcinoma (EOC). The secreted proteome of ascitic MO/MA was examined as an aid to the discovery of novel proteins in EOC that are likely to have biological relevance in the inflammatory pathways of EOC. Ascitic fluid MO/MA were isolated from EOC patients, grown short-term in serum-free media. MO/MA supernatants were analyzed for secreted proteins by HPLC fractionation followed by LC-tandem mass spectrometric analysis. The 14-3-3 zeta adaptor protein was identified in supernatants of three of three EOC patients but not in supernatants of buffy coat monocytes isolated from normal donors or the established monocyte cell line THP1. Moreover, 14-3-3 zeta was identified in ascitic fluids in eight of eight chemotherapy-naive patients by both immunoblot and mass spectrometric analysis. Immunofluorescent staining for 14-3-3 zeta demonstrated expression of the protein on ascitic and peritumoral macrophages in EOC patients. 14-3-3 zeta was also expressed on endothelial cells in the peritumoral stroma and partially on tumor cells. Uptake of 14-3-3 zeta was observed in EOC cell lines co-cultured with the recombinant protein expressed in E. coli. It is demonstrated for the first time that the important adaptor protein 14-3-3 zeta is common to the secretome of ascitic MO/MA and the ascites of advanced EOC patients.
- Subjects :
- Pathology
medicine.medical_specialty
Cancer Research
endocrine system diseases
Proteome
Cell
Immunology
Immunoblotting
Molecular Sequence Data
Fluorescent Antibody Technique
Buffy coat
Mass Spectrometry
Monocytes
Cell Line, Tumor
medicine
Immunology and Allergy
Humans
Amino Acid Sequence
Neoplasms, Glandular and Epithelial
Ovarian Neoplasms
biology
Monocyte
Macrophages
Signal transducing adaptor protein
Antibodies, Monoclonal
Ascites
medicine.disease
Molecular biology
female genital diseases and pregnancy complications
Secretory protein
medicine.anatomical_structure
Oncology
14-3-3 Proteins
Cell culture
biology.protein
Female
Antibody
Ovarian cancer
Sequence Alignment
Subjects
Details
- ISSN :
- 14320851
- Volume :
- 58
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cancer immunology, immunotherapy : CII
- Accession number :
- edsair.doi.dedup.....da88085d393f322ac9186d143cec7881