Back to Search
Start Over
Clinical Impact of Different Classes of Infiltrating T Cytotoxic and Helper Cells (Th1, Th2, Treg, Th17) in Patients with Colorectal Cancer
- Source :
- Cancer Research; Vol 71
- Publication Year :
- 2011
- Publisher :
- AMER ASSOC CANCER RESEARCH, 2011.
-
Abstract
- The tumor microenvironment includes a complex network of immune T-cell subpopulations. In this study, we systematically analyzed the balance between cytotoxic T cells and different subsets of helper T cells in human colorectal cancers and we correlated their impact on disease-free survival. A panel of immune related genes were analyzed in 125 frozen colorectal tumor specimens. Infiltrating cytotoxic T cells, Treg, Th1, and Th17 cells were also quantified in the center and the invasive margin of the tumors. By hierarchical clustering of a correlation matrix we identified functional clusters of genes associated with Th17 (RORC, IL17A), Th2 (IL4, IL5, IL13), Th1 (Tbet, IRF1, IL12Rb2, STAT4), and cytotoxicity (GNLY, GZMB, PRF1). Patients with high expression of the Th17 cluster had a poor prognosis, whereas patients with high expression of the Th1 cluster had prolonged disease-free survival. In contrast, none of the Th2 clusters were predictive of prognosis. Combined analysis of cytotoxic/Th1 and Th17 clusters improved the ability to discriminate relapse. In situ analysis of the density of IL17+ cells and CD8+ cells in tumor tissues confirmed the results. Our findings argue that functional Th1 and Th17 clusters yield opposite effects on patient survival in colorectal cancer, and they provide complementary information that may improve prognosis. Cancer Res; 71(4); 1263–71. ©2011 AACR.
- Subjects :
- Cancer Research
GZMB
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Immune system
Lymphocytes, Tumor-Infiltrating
Th2 Cells
GNLY
Medicine
Cytotoxic T cell
Humans
STAT4
Interleukin 4
Tumor microenvironment
business.industry
Gene Expression Profiling
Carcinoma
Forkhead Transcription Factors
T-Lymphocytes, Helper-Inducer
Th1 Cells
Microarray Analysis
Prognosis
Survival Analysis
3. Good health
Gene Expression Regulation, Neoplastic
Oncology
Tissue Array Analysis
030220 oncology & carcinogenesis
Immunology
Th17 Cells
Interleukin-4
business
Colorectal Neoplasms
CD8
030215 immunology
T-Lymphocytes, Cytotoxic
Subjects
Details
- Language :
- English
- ISSN :
- 00085472
- Volume :
- 71
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cancer Research
- Accession number :
- edsair.doi.dedup.....da5f62970abdefcb65154dee54b67fc5
- Full Text :
- https://doi.org/10.1158/0008-5472.CAN-10-2907