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Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared with Hypoxic Conditions
- Source :
- Stem Cells. 36:1226-1236
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Mesenchymal stem cells (MSCs) are commonly used in regenerative medicine, but their therapeutic effects vary depending on the culture environment. Hypoxic culturing can be used to maintain stem cells in an undifferentiated state, but is expensive and difficult to perform. The aim of this study was to determine the effectiveness of desferrioxamine (DFO), a hypoxia-mimetic reagent, as an alternative to hypoxic culturing by analyzing metabolic changes in MSCs under hypoxic conditions compared with changes induced by DFO. Low concentrations of DFO reduced mitochondrial activity and apoptosis. Therefore, low concentrations of DFO may be useful for MSC preconditioning. Metabolome analysis showed that both hypoxic treatment and DFO administration exhibited similar metabolite patterns except purine, pyrimidine, and tricarboxylic acid cycle (TCA) cycle related metabolites. Therefore, the use of DFO at low concentrations is a potential substitute for hypoxic culturing. These findings may form the foundation for the development of future regenerative therapies using MSCs.
- Subjects :
- Adult
Male
0301 basic medicine
Cell Survival
Glutamine
Metabolite
Apoptosis
Deferoxamine
Pharmacology
Biology
Regenerative medicine
03 medical and health sciences
chemistry.chemical_compound
Adenosine Triphosphate
Oxygen Consumption
Metabolomics
Nucleic Acids
medicine
Metabolome
Humans
Cell Proliferation
Principal Component Analysis
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Hypoxia (medical)
Hypoxia-Inducible Factor 1, alpha Subunit
Cell Hypoxia
Mitochondria
Up-Regulation
030104 developmental biology
Gene Expression Regulation
chemistry
Doxorubicin
Molecular Medicine
medicine.symptom
Stem cell
Developmental Biology
Subjects
Details
- ISSN :
- 15494918 and 10665099
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Stem Cells
- Accession number :
- edsair.doi.dedup.....da3b8bb1228fcddcaca6eb27bce69a20