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Characterization of genotypes among bovine viral diarrhea virus type 1 strains according to palindromic nucleotide substitutions in the genomic 5′-untranslated region
- Source :
- Journal of Virological Methods. 195:34-53
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Two-hundred and eighty-one strains of bovine viral diarrhea virus type 1 (BVDV-1) were evaluated according to the palindromic nucleotide substitutions (PNS) method, a simple and practical genotyping procedure, at the three variable loci, V1, V2 and V3, in the 5′-untranslated region of genomic RNA. Ten PNSs were characteristic to the Pestivirus genus, located in the V1 and V2 loci. The BVDV-1 species shared a U-A pairing, a species-characteristic PNS, in position fifteen common to the V1 locus, and a G-C pairing in position five common to the V2 and V3 loci. Within the BVDV-1 species, 15 genotypes, BVDV-1a, BVDV-1b, BVDV-1c, BVDV-1d, and the newly described from BVDV-1e to BVDV-1o were identified based on characteristic nucleotide base pairings. The nomenclature reflected the genotypes’ level of divergence within the species. Genotypes BVDV-1l, BVDV-1m and BVDV-1o were borderline in the species.
- Subjects :
- Genotype
Five prime untranslated region
animal diseases
viruses
Locus (genetics)
complex mixtures
Virology
Animals
Nucleotide
Base Pairing
Genotyping
Nomenclature
chemistry.chemical_classification
Genetics
biology
Diarrhea Virus 1, Bovine Viral
Pestivirus
Palindrome
Genetic Variation
virus diseases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
chemistry
Bovine Virus Diarrhea-Mucosal Disease
Cattle
5' Untranslated Regions
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 195
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....da3079663dde358c675ae79eb516a4d5