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Characterization of Odorant Receptors from a Non-ditrysian Moth, Eriocrania semipurpurella Sheds Light on the Origin of Sex Pheromone Receptors in Lepidoptera
- Source :
- Molecular Biology and Evolution
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Pheromone receptors (PRs) are essential in moths to detect sex pheromones for mate finding. However, it remains unknown from which ancestral proteins these specialized receptors arose. The oldest lineages of moths, so-called non-ditrysian moths, use short-chain pheromone components, secondary alcohols, or ketones, so called Type 0 pheromones that are similar to many common plant volatiles. It is, therefore, possible that receptors for these ancestral pheromones evolved from receptors detecting plant volatiles. Hence, we identified the odorant receptors (ORs) from a non-ditrysian moth, Eriocrania semipurpurella (Eriocraniidae, Lepidoptera), and performed functional characterization of ORs using HEK293 cells. We report the first receptors that respond to Type 0 pheromone compounds; EsemOR3 displayed highest sensitivity toward (2S, 6Z)-6-nonen-2-ol, whereas EsemOR5 was most sensitive to the behavioral antagonist (Z)-6-nonen-2-one. These receptors also respond to plant volatiles of similar chemical structures, but with lower sensitivity. Phylogenetically, EsemOR3 and EsemOR5 group with a plant volatile-responding receptor from the tortricid moth Epiphyas postvittana (EposOR3), which together reside outside the previously defined lepidopteran PR clade that contains the PRs from more derived lepidopteran families. In addition, one receptor (EsemOR1) that falls at the base of the lepidopteran PR clade, responded specifically to β-caryophyllene and not to any other additional plant or pheromone compounds. Our results suggest that PRs for Type 0 pheromones have evolved from ORs that detect structurally-related plant volatiles. They are unrelated to PRs detecting pheromones in more derived Lepidoptera, which, in turn, also independently may have evolved a novel function from ORs detecting plant volatiles.
- Subjects :
- 0301 basic medicine
sex pheromone
Moths
Receptors, Odorant
Pheromones
odorant receptor
Evolution, Molecular
Lepidoptera genitalia
03 medical and health sciences
Eriocrania semipurpurella
Botany
Genetics
Animals
Humans
Sex Attractants
Receptor
deorphanization
Molecular Biology
Phylogeny
Ecology, Evolution, Behavior and Systematics
Polycyclic Sesquiterpenes
Errata
biology
fungi
Ketones
biology.organism_classification
Receptors, Pheromone
Lepidoptera
HEK293 Cells
030104 developmental biology
Sex pheromone
Eriocraniidae
Fast Track
Pheromone
Carrier Proteins
Sesquiterpenes
Function (biology)
Subjects
Details
- ISSN :
- 15371719 and 07374038
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Molecular Biology and Evolution
- Accession number :
- edsair.doi.dedup.....da239004f4c46923e613b14270bb9a6c