Back to Search
Start Over
MicroRNA-379-5p is associated with biochemical premature ovarian insufficiency through PARP1 and XRCC6
- Source :
- Cell Death and Disease, Vol 9, Iss 2, Pp 1-10 (2018), Cell Death & Disease
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group, 2018.
-
Abstract
- Premature ovarian insufficiency (POI) imposes great challenges on women’s fertility and lifelong health. POI is highly heterogeneous and encompasses occult, biochemical, and overt stages. MicroRNAs (miRNAs) are negative regulators of gene expression, whose roles in physiology and diseases like cancers and neurological disorders have been recognized, but little is known about the miRNAs profile and functional relevance in biochemical POI (bPOI). In this study, the expression of miRNAs and mRNAs in granulosa cells (GCs) of bPOI women was determined by two microarrays, respectively. MiR-379-5p, PARP1, and XRCC6 were differentially expressed in GCs of bPOI as revealed by microarrays. Subsequently, functional studies demonstrated that miR-379-5p overexpression inhibited granulosa cell proliferation and attenuated DNA repair efficiency. Furthermore, both PARP1 and XRCC6 showed lower levels in GCs from patients with bPOI and were identified as executives of miR-379-5p. Therefore, our data first uncovered potentially pathogenic miR-379-5p and two novel targets PARP1 and XRCC6 in bPOI, which corroborated the significance of DNA repair for POI, and brought up an epigenetic explanation for the disease.
- Subjects :
- 0301 basic medicine
Adult
Cancer Research
DNA Repair
DNA repair
Immunology
Poly (ADP-Ribose) Polymerase-1
Biology
Primary Ovarian Insufficiency
Bioinformatics
Premature ovarian insufficiency
Article
03 medical and health sciences
Cellular and Molecular Neuroscience
microRNA
Humans
Epigenetics
lcsh:QH573-671
Granulosa cell proliferation
Ku Autoantigen
Cell Proliferation
Regulation of gene expression
Granulosa Cells
Base Sequence
lcsh:Cytology
Gene Expression Profiling
Cell Biology
Gene expression profiling
MicroRNAs
030104 developmental biology
Gene Expression Regulation
Case-Control Studies
Female
DNA microarray
Subjects
Details
- Language :
- English
- ISSN :
- 20414889
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cell Death and Disease
- Accession number :
- edsair.doi.dedup.....da1f3e431864ad680945d1cfb2ddd7fa