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At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media
- Source :
- Applied Microbiology and Biotechnology
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Spore inoculum quality in filamentous bioprocesses is a critical parameter associated with viable spore concentration (1) and spore germination (2). It influences pellet morphology and, consequently, process performance. The state-of-the-art method to measure viable spore concentration is tedious, associated with significant inherent bias, and not applicable in real-time. Therefore, it is not usable as process analytical technology (PAT). Spore germination has so far been monitored using image analysis, which is hampered by complex medium background often observed in filamentous bioprocesses. The method presented here is based on the combination of viability staining and large-particle flow cytometry which enables measurements in real-time and hence aims to be applicable as a PAT tool. It is compatible with the complex media background and allows the quantification of metabolically active spores and the monitoring of spore germination. A distinction of germinated spores and not germinated spores was based on logistic regression, using multiparameteric data from flow cytometry. In a first step, a significant correlation between colony-forming unit (CFU) counts and viable spore concentration (1) in an industrially relevant model bioprocess was found. Spore germination (2) was followed over the initial process phase with close temporal resolution. The validation of the method showed an error below 5 %. Differences in spore germination for various spore inocula ages and spore inoculum concentrations were monitored. The real-time applicability of the method suggests the implementation as a PAT tool in filamentous bioprocesses. Electronic supplementary material The online version of this article (doi:10.1007/s00253-016-7787-y) contains supplementary material, which is available to authorized users.
- Subjects :
- Microbiological Techniques
0106 biological sciences
0301 basic medicine
Filamentous fungi
Penicillium chrysogenum
Process analytical technology
01 natural sciences
Applied Microbiology and Biotechnology
03 medical and health sciences
010608 biotechnology
Botany
Spore germination
Flow cytometry
Food science
Spore viability
Bioprocess
Microbial Viability
Staining and Labeling
biology
Methods and Protocols
fungi
General Medicine
Spores, Fungal
biology.organism_classification
Culture Media
Spore
Viability staining
030104 developmental biology
Critical parameter
Germination
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....da1e8e2bbf3b17e97fb025b9fddf69c8