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Design and Synthesis of a Novel Colorimetric Fluorescent Probe for the Selective Detection of Sulfur Dioxide in SH-SY5Y Neuroblastoma Cells and Its Applications in Traditional Chinese Medicines
- Source :
- New Journal of Chemistry, New Journal of Chemistry, Royal Society of Chemistry, 2019, 43 (10), pp.4188--4195. ⟨10.1039/C8NJ06326E⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- A fast response time, low limit of detection and high fluorescence quantum yield probe DTCC was designed and synthesized to detect SO 2 derivatives based on coumarin-thiophene dye which was fused with a coumarin moiety and 2-thiophenecarboxaldehyde. , Sulfur fumigation has attracted extensive attention as one of the important post-harvest processing methods for some traditional Chinese medicines (TCMs) in the last decade. However, sulfur fumigation has recently emerged as a controversial topic due to its potential detrimental effects on the safety and efficacy of TCMs as some sulfur-fumigated TCMs contain numerous sulfur dioxide derivatives; moreover, high levels of sulfur dioxide derivatives can cause some diseases and dangerous environmental pollution. In this study, a DTCC probe with a fast response time, low limit of detection and a high fluorescence quantum yield was designed and synthesized to detect SO 2 derivatives based on the coumarin-thiophene dye, which was fused with a coumarin moiety and 2-thiophenecarboxaldehyde. The probe DTCC exhibited a fast response time (less than 10 s), satisfactory selectivity for SO 2 derivatives in the presence of other ROS and excellent sensitivity for SO 2 derivatives with a low limit of detection (0.23 μM) as well as a wide linear range (0\textendash 100 μM). Furthermore, the probe DTCC was successfully applied in fluorescence imaging in SH-SY5Y neuroblastoma cells with excellent membrane permeability and stability. It was also employed in monitoring the total SO 2 derivatives in several real TCM samples. These results illustrate that the probe DTCC has an excellent capability for monitoring SO 2 derivatives in living cells and real TCM samples.
- Subjects :
- Detection limit
Fluorescence-lifetime imaging microscopy
Membrane permeability
POLE 3
chemistry.chemical_element
Environmental pollution
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Fluorescence
Sulfur
Combinatorial chemistry
Catalysis
0104 chemical sciences
chemistry.chemical_compound
GOBS
chemistry
Linear range
13. Climate action
Materials Chemistry
[CHIM]Chemical Sciences
0210 nano-technology
Sulfur dioxide
Subjects
Details
- Language :
- English
- ISSN :
- 11440546 and 13699261
- Database :
- OpenAIRE
- Journal :
- New Journal of Chemistry, New Journal of Chemistry, Royal Society of Chemistry, 2019, 43 (10), pp.4188--4195. ⟨10.1039/C8NJ06326E⟩
- Accession number :
- edsair.doi.dedup.....da119a4c572dea9ad9b16a7f92fa3af8