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Non-Cationic Proteins Are Associated with HIV Neutralizing Activity in Genital Secretions of Female Sex Workers
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 6, p e0130404 (2015)
- Publication Year :
- 2015
- Publisher :
- Public Library of Science, 2015.
-
Abstract
- Objective Cationic proteins found in cervicovaginal secretions (CVS) are known to contribute to the early antiviral immune response against HIV-infection in vitro. We here aimed to define additional antiviral factors that are over-expressed in CVS from female sex workers at high risk of infection. Methods CVS were collected from Kenyan HIV-seronegative (n = 34) and HIV-seropositive (n = 12) female sex workers, and were compared with those from HIV-seronegative low-risk women (n = 12). The highly exposed seronegative (HESN) sex workers were further divided into those with less (n = 22) or more (n = 12) than three years of documented sex work. Cationic protein-depleted CVS were assessed for HIV-neutralizing activity by a PBMC-based HIV-neutralizing assay, and then characterized by proteomics. Results HIV neutralizing activity was detected in all unprocessed CVS, however only CVS from the female sex worker groups maintained its HIV neutralizing activity after cationic protein-depletion. Differentially abundant proteins were identified in the cationic protein-depleted secretions including 26, 42, and 11 in the HESN>3yr, HESN
- Subjects :
- Bodily Secretions
lcsh:Medicine
HIV Infections
Biology
Peripheral blood mononuclear cell
Immune system
medicine
Humans
Sex organ
Mode of action
lcsh:Science
Multidisciplinary
Sex Workers
lcsh:R
Proteins
Correction
Protective Factors
Virology
Phenotype
In vitro
Immunity, Innate
medicine.anatomical_structure
Membrane protein
Immunology
Vagina
Leukocytes, Mononuclear
lcsh:Q
Female
Disease Susceptibility
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....da0429f46e7331dd4c0c5508e179266e