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The beta-tubulin genes of two Strongyloides species
- Source :
- Experimental parasitology. 112(3)
- Publication Year :
- 2005
-
Abstract
- The World Health Organization is sponsoring major treatment programs with the aim of controlling helminth infection throughout the tropical world. Prominent among the anthelmintics recommended for use in these programs are drugs in the benzimidazole (BZ) class. Resistance to these drugs has been associated with polymorphisms in the beta-tubulin gene. We have cloned and sequenced the beta-tubulin genes of Strongyloides stercoralis and Strongyloides ratti and have proceeded to develop a protocol for genotyping single worms for polymorphisms in beta-tubulin. Our findings indicate that S. ratti has a single beta-tubulin gene, making DNA sequence analysis of a single larva PCR product a feasible means of studying BZ resistance in these species. Our genotyping test allows the identification of polymorphisms at codons 167, 198, and 200 in the Strongyloides beta-tubulin gene, thus enabling survey for BZ resistant genotypes.
- Subjects :
- DNA, Complementary
Genotype
Sequence analysis
Immunology
Molecular Sequence Data
Drug Resistance
Helminth genetics
Polymerase Chain Reaction
Strongyloides stercoralis
law.invention
law
Tubulin
parasitic diseases
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Rats, Wistar
Gene
Genotyping
3' Untranslated Regions
Polymerase chain reaction
DNA Primers
Genetics
Electrophoresis, Agar Gel
Polymorphism, Genetic
biology
Base Sequence
Strongyloides ratti
General Medicine
DNA, Helminth
biology.organism_classification
Rats
Blotting, Southern
Infectious Diseases
Strongyloides
Parasitology
Benzimidazoles
RNA, Helminth
Sequence Alignment
Subjects
Details
- ISSN :
- 00144894
- Volume :
- 112
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Experimental parasitology
- Accession number :
- edsair.doi.dedup.....d9fa19329ca157066df45fb91eb7d767