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Human γδ T cells recognize CD1b by two distinct mechanisms
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, 117(37), 22944-22952. NATL ACAD SCIENCES, Proceedings of the National Academy of Sciences of the United States of America, 117(37), 22944. National Academy of Sciences
- Publication Year :
- 2020
- Publisher :
- Proceedings of the National Academy of Sciences, 2020.
-
Abstract
- γδ T cells form an abundant part of the human cellular immune system, where they respond to tissue damage, infection, and cancer. The spectrum of known molecular targets recognized by Vδ1-expressing γδ T cells is becoming increasingly diverse. Here we describe human γδ T cells that recognize CD1b, a lipid antigen-presenting molecule, which is inducibly expressed on monocytes and dendritic cells. Using CD1b tetramers to study multiple donors, we found that many CD1b-specific γδ T cells use Vδ1. Despite their common use of Vδ1, three CD1b-specific γδ T cell receptors (TCRs) showed clear differences in the surface of CD1b recognized, the requirement for lipid antigens, and corecognition of butryophilinlike proteins. Several Vγ segments were present among the CD1bspecific TCRs, but chain swap experiments demonstrated that CD1b specificity was mediated by the Vδ1 chain. One of the CD1b-specific Vδ1+ TCRs paired with Vγ4 and shows dual reactivity to CD1b and butyrophilin-like proteins. αβ TCRs typically recognize the peptide display platform of MHC proteins. In contrast, our results demonstrate the use of rearranged receptors to mediate diverse modes of recognition across the surface of CD1b in ways that do and do not require carried lipids.
- Subjects :
- Model organisms
Models, Molecular
T-Lymphocytes
Immunology
CD1b
Crystallography, X-Ray
Lymphocyte Activation
Major histocompatibility complex
Monocytes
Antigens, CD1
Immune system
Tissue damage
Humans
Receptor
Intraepithelial Lymphocytes
Vd1
Butyrophilin-like molecule
Human Biology & Physiology
Antigen Presentation
Multidisciplinary
Peptide display
biology
lipid antigen
FOS: Clinical medicine
Lipid antigen
T-cell receptor
butyrophilin-like molecule
gd T cell
Lipids
Cell biology
Molecular targets
biology.protein
Gd T cell
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....d9f8af35656962fe3d93fad9047aeb7b