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Macrocyclic lactone anthelmintic-induced leukocyte binding to Dirofilaria immitis microfilariae: Influence of the drug resistance status of the parasite
- Source :
- International Journal for Parasitology: Drugs and Drug Resistance, Vol 10, Iss, Pp 45-50 (2019), International Journal for Parasitology: Drugs and Drug Resistance
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- The macrocyclic lactone anthelmintics are the only class of drug currently used to prevent heartworm disease. Their extremely high potency in vivo is not mirrored by their activity against Dirofilaria immitis larvae in vitro, leading to suggestions that they may require host immune functions to kill the parasites. We have previously shown that ivermectin stimulates the binding of canine peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs) to D. immitis microfilariae (Mf). We have now extended these studies to moxidectin and examined the ability of both drugs to stimulate canine PBMC and PMN attachment to Mf from multiple strains of D. immitis, including two that are proven to be resistant to ivermectin in vivo. Both ivermectin and moxidectin significantly increased the percentage of drug-susceptible parasites with cells attached at very low concentrations (100 nM) were required to increase the percentage of the two resistant strains, Yazoo-2013 and Metairie-2014, with cells attached. Moxidectin increased the percentage of the two resistant strains with cells attached at lower concentrations (<br />Graphical abstract Image 1<br />Highlights • Ivermectin promotes attachment of PMN and PBMC to D. immitis microfilariae in vitro. • Moxidectin has a similar effect. • Higher ivermectin concentrations are needed if Mf of ML-resistant strains are used. • Moxidectin is more effective at promoting cell attachment to resistant Mf. • Neither PMN nor PBMC attachment does not result in parasite death in vitro.
- Subjects :
- Male
0301 basic medicine
Polymorphonuclear leukocytes
Dirofilaria immitis
030231 tropical medicine
Drug Resistance
Drug resistance
Article
Moxidectin
Microbiology
lcsh:Infectious and parasitic diseases
Lactones
03 medical and health sciences
chemistry.chemical_compound
Dogs
0302 clinical medicine
Immune system
Ivermectin
In vivo
parasitic diseases
Cell Adhesion
medicine
Animals
Potency
Pharmacology (medical)
lcsh:RC109-216
Dog Diseases
Anthelmintic
Microfilariae
Anthelmintics
Pharmacology
biology
biology.organism_classification
3. Good health
030104 developmental biology
Infectious Diseases
chemistry
Peripheral blood mononuclear cells
Leukocytes, Mononuclear
Female
Parasitology
Dirofilariasis
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 22113207
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal for Parasitology: Drugs and Drug Resistance
- Accession number :
- edsair.doi.dedup.....d9f2f36aa83fb978c304d1d6b8c98fbf