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Extraction and characterization of novel multifunctional peptides from Trachinus Draco (greater weever) myofibrillar proteins with ACE/DPP4 inhibitory, antioxidant, and metal chelating activities
- Source :
- Journal of Food Biochemistry. 44
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- Marine organisms represent a great source of natural bioactive molecules, from which bioactive peptides are of great importance in biomedicine application in many diseases such as diabetes and its related complications. in this study, greater weever (Trachinus Draco) myofibrillar proteins were sequentially hydrolyzed and the different RP-HPLC purified fractions were tested for potential inhibitory activities of ACE and DPP4, in addition to metal chelation and antioxidant activities. Four fractions were found to have high levels of activity (with two peptides being multifunctional) and were subsequently sequenced using the de novo sequencing method. the results indicate that the peptides are novel and highly effective for each related activity compared to reference molecules. the current findings suggest these multifunctional peptides as promising therapeutics against oxidative stress, hypertension, and diabetes. Practical applications We have described the finding of two multifunctional bioactive peptides from Trachinus Draco (greater weever) myofibrillar proteins having two or more activities. They have ACE inhibitory, DPP4 inhibitory, antioxidant, and metal chelation activities. These new peptides could be used for future biomedicine applications as a stand-alone treatment, in combination with other molecules, or as a supplement. Furthermore, after identification of their sequence in our work, it would have a great potential to be artificially synthesized. the field of food supplements could be explored further.<br />Turkiye Bilimsel ve Teknolojik Arastirma Kurumu [Short Term R and D Funding Program (1002), 215Z486] Funding Source: Medline
- Subjects :
- multifunctional peptides
Metal chelating activity
Antioxidant
030309 nutrition & dietetics
Dipeptidyl Peptidase 4
DPP4 inhibition
medicine.medical_treatment
Biophysics
antioxidant activity
Inhibitory postsynaptic potential
medicine.disease_cause
Antioxidants
03 medical and health sciences
Hydrolysis
0404 agricultural biotechnology
metal chelating activity
medicine
Animals
Chelation
Trachinus Draco
Trachinus draco
Pharmacology
Chromatography, Reverse-Phase
0303 health sciences
biology
Chemistry
ACE inhibition
04 agricultural and veterinary sciences
Cell Biology
biology.organism_classification
040401 food science
Perciformes
Biochemistry
Peptides
Myofibril
Oxidative stress
Food Science
Subjects
Details
- ISSN :
- 17454514 and 01458884
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Food Biochemistry
- Accession number :
- edsair.doi.dedup.....d9d0ad7aea02d74f0de0c874cc1ed43e