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Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 2, p e0172724 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role for VGF was supported by observations in a VGF null (VGF-/-) mouse and in the diet-induced and gold-thioglucose obese mice. However, this anabolic role for VGF has not been supported by a number of subsequent studies investigating the physiological effects of VGF-derived peptides. Intracerebroventricular (ICV) infusion of TLQP-21 increased resting energy expenditure and rectal temperature in mice and protected against diet-induced obesity. Similarly, ICV infusion of TLQP-21 into Siberian hamsters significantly reduced body weight, but this was due to a decrease in food intake, with no effect on energy expenditure. Subsequently NERP-2 was shown to increase food intake in rats via the orexin system, suggesting opposing roles for these VGF-derived peptides. Thus to further elucidate the role of hypothalamic VGF in the regulation of energy homeostasis we utilised a recombinant adeno-associated viral vector to over-express VGF in adult male Siberian hamsters, thus avoiding any developmental effects or associated functional compensation. Initially, hypothalamic over-expression of VGF in adult Siberian hamsters produced no effect on metabolic parameters, but by 12 weeks post-infusion hamsters had increased oxygen consumption and a tendency to increased carbon dioxide production; this attenuated body weight gain, reduced interscapular white adipose tissue and resulted in a compensatory increase in food intake. These observed changes in energy expenditure and food intake were associated with an increase in the hypothalamic contents of the VGF-derived peptides AQEE, TLQP and NERP-2. The complex phenotype of the VGF-/- mice is a likely consequence of global ablation of the gene and its derived peptides during development, as well as in the adult.
- Subjects :
- 0301 basic medicine
Anabolism
Physiology
Epidemiology
lcsh:Medicine
Mice, Obese
White adipose tissue
Disease Vectors
Weight Gain
Biochemistry
Energy homeostasis
Eating
Mice
Cricetinae
Brown adipose tissue
Medicine and Health Sciences
Biomechanics
lcsh:Science
Siberian hamsters, VGF, hypothalamus, adeno-associated viral virus
Mammals
Multidisciplinary
Respiration
Brain
medicine.anatomical_structure
Physiological Parameters
Adipose Tissue
Hypothalamus
Vertebrates
Hamsters
Brown Adipose Tissue
Anatomy
Viral Vectors
Research Article
medicine.medical_specialty
Phodopus
Hamster
Nerve Tissue Proteins
Bioenergetics
Biology
Rodents
Microbiology
03 medical and health sciences
Oxygen Consumption
Virology
Internal medicine
medicine
Animals
Resting energy expenditure
Obesity
Biological Locomotion
lcsh:R
Body Weight
Neuropeptides
Organisms
Biology and Life Sciences
Correction
Peptide Fragments
Orexin
Rats
Biological Tissue
030104 developmental biology
Endocrinology
Gene Expression Regulation
Amniotes
lcsh:Q
Physiological Processes
Energy Metabolism
Viral Transmission and Infection
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 12, Iss 2, p e0172724 (2017)
- Accession number :
- edsair.doi.dedup.....d98ab117c57c14eb43a0771a89db9a30