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Optical imaging of ovarian cancer using a matrix metalloproteinase-3-sensitive near-infrared fluorescent probe
- Source :
- PLoS ONE, Vol 13, Iss 2, p e0192047 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Epithelial ovarian cancer (EOC) is the seventh most common cancer among women worldwide. The 5-year survival rate for women with EOC is only 30%-50%, which is largely due to the typically late diagnosis of this condition. EOC is difficult to detect in its early stage because of its asymptomatic nature. Recently, near-infrared fluorescent (NIRF) imaging has been developed as a potential tool for detecting EOC at the molecular level. In this study, a NIRF-sensitive probe was designed to detect matrix metalloproteinase (MMP) activity in ovarian cancer cells. A cyanine fluorochrome was conjugated to the amino terminus of a peptide substrate with enzymatic specificity for MMP-3. To analyze the novel MMP-3 probe, an in vivo EOC model was established by subcutaneously implanting SKOV3 cells, a serous-type EOC cell line, in mice. This novel MMP-3-sensitive probe specifically reacted with only the active MMP-3 enzyme, resulting in a significantly enhanced NIRF emission intensity. Histological analysis demonstrated that MMP-3 expression and activity were enhanced in the stromal cells surrounding the ovarian cancer cells. These studies establish a molecular imaging reporter for diagnosing early-stage EOC. Additional studies are required to confirm the early-stage activity of MMP-3 in EOC and its diagnostic and prognostic significance.
- Subjects :
- 0301 basic medicine
Fluorescence-lifetime imaging microscopy
endocrine system diseases
lcsh:Medicine
Biochemistry
Diagnostic Radiology
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
lcsh:Science
Connective Tissue Cells
Liquid Chromatography
Metalloproteinase
Multidisciplinary
Chemistry
Radiology and Imaging
Chromatographic Techniques
Proteases
Magnetic Resonance Imaging
female genital diseases and pregnancy complications
Ovarian Cancer
Enzymes
In Vivo Imaging
Oncology
Connective Tissue
030220 oncology & carcinogenesis
Cellular Types
Anatomy
Preclinical imaging
Research Article
Stromal cell
Imaging Techniques
Research and Analysis Methods
03 medical and health sciences
In vivo
Diagnostic Medicine
Fluorescence Imaging
medicine
Cancer Detection and Diagnosis
lcsh:R
Cancer
Cancers and Neoplasms
Biology and Life Sciences
Proteins
Cell Biology
medicine.disease
High Performance Liquid Chromatography
030104 developmental biology
Biological Tissue
Cancer research
Enzymology
Metalloproteases
lcsh:Q
Molecular imaging
Stromal Cells
Ovarian cancer
Gynecological Tumors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d9728ef3e823cb95c2f90e16f0c382c6