Back to Search
Start Over
Novelty and spatio–temporal heterogeneity in the bacterial diversity of hypersaline Lake Tebenquiche (Salar de Atacama)
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- 14 pages, 7 figures, 3 tables<br />Lake Tebenquiche is one of the largest saline water bodies in the Salar de Atacama at 2,500 m above sea level in northeastern Chile. Bacteria inhabiting there have to deal with extreme changes in salinity, temperature and UV dose (i.e., high environmental dissimilarity in the physical landscape). We analyzed the bacterioplankton structure of this lake by 16S rRNA gene analyses along a spatio–temporal survey. The bacterial assemblage within the lake was quite heterogeneous both in space and time. Salinity changed both in space and time ranging between 1 and 30% (w/v), and total abundances of planktonic prokaryotes in the different sampling points within the lake ranged between two and nine times 106 cells mL−1. Community composition changed accordingly to the particular salinity of each point as depicted by genetic fingerprinting analyses (denaturing gradient gel electrophoresis), showing a high level of variation in species composition from place to place (beta-diversity). Three selected sites were analyzed in more detail by clone libraries. We observed a predominance of Bacteroidetes (about one third of the clones) and Gammaproteobacteria (another third) with respect to all the other bacterial groups. The diversity of Bacteroidetes sequences was large and showed a remarkable degree of novelty. Bacteroidetes formed at least four clusters with no cultured relatives in databases and rather distantly related to any known 16S rRNA sequence. Within this phylum, a rich and diverse presence of Salinibacter relatives was found in the saltiest part of the lake. Lake Tebenquiche included several novel microorganisms of environmental importance and appeared as a large unexplored reservoir of unknown bacteria<br />Sampling and measurements carried out in Chile were funded by grants FONDECYT 1030441 and FONDEF D99I1026. Measurements carried out in Barcelona were funded by grant "ATACAMA-2002" (CICYT, BOS2002-10258-E). Grant "BIOARSENICO" from Fundación BBVA funds current work
- Subjects :
- Ultraviolet Rays
Tebenquiche
Models, Biological
Microbiology
Extremophiles
RNA, Ribosomal, 16S
Gammaproteobacteria
Extremophile
Chile
16S rRNA
DGGE
Fingerprinting
Clone libraries
Bacteria
Geography
Ecology
biology
Bacteroidetes
Temperature
Genetic Variation
Equipment Design
Sequence Analysis, DNA
Biodiversity
General Medicine
Bacterioplankton
Hypersaline lake
Hydrogen-Ion Concentration
Salar
Plankton
biology.organism_classification
Salt lake
Salinity
Microscopy, Fluorescence
RNA, Ribosomal
Hypersaline
Molecular Medicine
Salts
Water Microbiology
Temperature gradient gel electrophoresis
Subjects
Details
- ISSN :
- 14334909 and 14310651
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Extremophiles
- Accession number :
- edsair.doi.dedup.....d96f8c3dc10b45a05fd206d80e2bf55a