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Natural history of fetal cell microchimerism during and following murine pregnancy
- Source :
- Journal of Reproductive Immunology. 66:1-12
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- In humans, fetal cells enter the maternal circulation during all pregnancies and can persist for decades. Human studies, however, are often limited by the number of subjects and the availability of healthy and diseased tissues for analysis. We sought to develop a murine model to establish the natural history of fetal cell microchimerism in various maternal tissues during and after healthy pregnancies resulting from congenic and allogenic matings. We bred C57BL/6J and DBA/2J virgin female mice to C57BL/6J males transgenic for the enhanced green fluorescent protein (GFP), which shows autosomal dominant inheritance with complete penetrance and is under the control of a ubiquitous chicken beta-actin promoter and a cytomegalovirus enhancer. During pregnancy and at different times after delivery, female mice were sacrificed. Tissues were collected and the presence of the gfp transgene and GFP+ cells was assessed by real-time quantitative PCR and by immunofluorescence. During pregnancy, microchimerism was detected in all tissues from mice carrying GFP+ fetuses. Fetal cells were often mononuclear. The frequency of fetal cells in the lungs was significantly higher compared to other tissues. The level of microchimerism was also significantly higher in congenic compared to allogenic matings. After delivery, the frequency of fetal cells decreased and fetal cells were undetectable at 2 and 3 weeks after the first delivery. However, some mice that had three gestations had detectable fetal cells 3 weeks after their last delivery. Using sensitive methods of detection, we demonstrate that fetal cell microchimerism occurs during all murine pregnancies. We describe a useful model for the study of the consequences of this phenomenon.
- Subjects :
- Male
Transgene
Green Fluorescent Proteins
Immunology
Congenic
Penetrance
Biology
Immunofluorescence
Chimerism
Green fluorescent protein
Andrology
Mice
Fetus
Pregnancy
medicine
Animals
Immunology and Allergy
Transgenes
Promoter Regions, Genetic
Lung
medicine.diagnostic_test
Obstetrics and Gynecology
Microchimerism
medicine.disease
Actins
Reproductive Medicine
Models, Animal
Gestation
Female
Subjects
Details
- ISSN :
- 01650378
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Journal of Reproductive Immunology
- Accession number :
- edsair.doi.dedup.....d95dd9edb470a626a7d041acd3734078