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Drill-assisted genomic DNA extraction from Botrytis cinerea
- Source :
- Biotechnology Letters. 30:1989-1992
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- Current DNA extraction protocols for genomic DNA from Botrytis cinerea almost always start with mycelium that has been reduced to powder with liquid N(2) in a mortar, and this makes their application to a large number of samples slow and cumbersome. Here we present an adaptation of an existing method [Möller et al. (1992) Nucleic Acids Res 20: 6115-6116] for which the initial steps have been modified, including the homogenization of the fungus with sand and the aid of a common household drill. This method allows the processing of large number of samples in much shorter times and generates an average of 4 mug DNA per sample, of sufficient quality for use in PCR and Southern blotting.
- Subjects :
- Chromatography
Mycelium
Reproducibility of Results
Bioengineering
General Medicine
Biology
biology.organism_classification
Applied Microbiology and Biotechnology
Molecular biology
DNA extraction
law.invention
genomic DNA
chemistry.chemical_compound
chemistry
law
Nucleic acid
Botrytis
DNA, Fungal
Molecular Biology
DNA
Polymerase chain reaction
Biotechnology
Southern blot
Botrytis cinerea
Subjects
Details
- ISSN :
- 15736776, 01415492, and 61156116
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....d93c2f8b93891fcb6d2307b268ee65a3