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The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins
- Source :
- Nucleic Acids Research. 24:3821-3828
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- The murine poly(C)-binding protein (mCBP) was previously shown to belong to the group of K-homology (KH) proteins by virtue of its homology to hnRNP-K. We have isolated cDNA-splice variants of mCBP which differ by two variable regions of 93 bp and/or 39 +/- 3 bp respectively. Both variable regions are located between the second and third KH-domain of mCBP. The characterization of a partial genomic clone enabled us to propose a model for the generation of the second variable region by the use of a putative alternative splice signal. The mCBP mRNA is expressed ubiquitously and the protein is found predominantly in the nucleus with the exception of the nucleoli. We have identified five proteins which interact with mCBP in the yeast two hybrid system: mouse y-box protein 1 (msy-1), y-box-binding protein, hnRNP-L, filamin and splicing factor 9G8. The interaction between mCBP and splicing factor 9G8 was confirmed in vivo. These results suggest a function of mCBP in RNA metabolism.
- Subjects :
- Nucleocytoplasmic Transport Proteins
RNA Splicing
Two-hybrid screening
Molecular Sequence Data
RNA-binding protein
Saccharomyces cerevisiae
Biology
Mice
Splicing factor
Isomerism
Genetics
Animals
Nuclear protein
Messenger RNA
Serine-Arginine Splicing Factors
Binding protein
Nuclear Proteins
RNA-Binding Proteins
RNA
Molecular biology
Poly C
Biochemistry
RNA splicing
Pseudogenes
Protein Binding
Subcellular Fractions
Research Article
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....d9214b37bc93acaeb2e56a780e32181c