Short halt in vaping modifies cardio-respiratory parameters and urine metabolome: a randomized trial

Background: Propylene glycol and glycerol are e-cigarette constituents that facilitate liquid vaporization and nicotine transport. As these small hydrophilic molecules quickly cross the lung epithelium, we hypothesized that short-term cessation of vaping in regular users would completely clear aerosol deposit from the lungs and reverse vaping-induced cardio-respiratory toxicity. Aims: To assess the acute effects of vaping and their reversibility on biological/clinical cardio-respiratory parameters (serum/urine pneumoproteins, hemodynamic parameters, lung-function test and diffusing capacities, transcutaneous gas tensions (primary outcome) and skin microcirculatory blood flow). Methods: Regular e-cigarette users were enrolled in this randomized, investigator-blinded, three-period crossover-study. The periods consisted of nicotine-vaping (nicotine-session), nicotine-free vaping (nicotine-free-session), and complete cessation of vaping (stop-session), all maintained for five days before the session began. Multiparametric metabolomic analyses were used to verify subjects' protocol compliance. Biological/clinical cardio-respiratory parameters were assessed at the beginning of each session (baseline) and after acute vaping exposure. Results: Compared to the nicotine- and nicotine-free-sessions, a specific metabolomic signature characterized the stop-session. Baseline serum club-cell protein-16 was higher during the stop-session than the other sessions ( p
info:eu-repo/semantics/published