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Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke
- Source :
- Theoretical and Applied Genetics, 118(8), 1573-1587, Theoretical and Applied Genetics 118 (2009) 8
- Publication Year :
- 2009
-
Abstract
- Cynara cardunculus includes three taxa, the globe artichoke (subsp. scolymus L. Hegi), the cultivated cardoon (var. altilis) and their progenitor, the wild cardoon (var. sylvestris). Globe artichoke is an important component of the Mediterranean rural economy, but its improvement through breeding has been rather limited and its genome organization remains largely unexplored. Here, we report the isolation of 61 new microsatellite loci which amplified a total of 208 alleles in a panel of 22 C. cardunculus genotypes. Of these, 51 were informative for linkage analysis and 39 were used to increase marker density in the available globe artichoke genetic maps. Sequence analysis of the 22 loci associated with genes showed that 9 are located within coding sequence, with the repetitive domain probably being involved in DNA binding or in protein-protein interactions. The expression of the genes associated with 9 of the 22 microsatellite loci was demonstrated by RT-PCR.
- Subjects :
- Genetic Markers
dna-sequences
DNA, Plant
Genotype
Sequence analysis
Genetic Linkage
Molecular Sequence Data
scolymus l
Genomics
cynara-cardunculus l
Genome
DNA sequencing
Chromosomes, Plant
Gene mapping
Genetic linkage
PRI Biodiversiteit en Veredeling
Cynara scolymus
Genetics
cultured rat hepatocytes
Amino Acid Sequence
Alleles
Genomic Library
Polymorphism, Genetic
biology
Base Sequence
Sequence Homology, Amino Acid
Cynara
Chromosome Mapping
General Medicine
DNA
Sequence Analysis, DNA
biology.organism_classification
linkage map
PRI Biodiversity and Breeding
fragment length polymorphisms
mediterranean environment
potential source
transcription factor-binding
Microsatellite
Agronomy and Crop Science
Genome, Plant
medicago-truncatula
Biotechnology
Microsatellite Repeats
Subjects
Details
- Language :
- English
- ISSN :
- 00405752
- Database :
- OpenAIRE
- Journal :
- Theoretical and Applied Genetics, 118(8), 1573-1587, Theoretical and Applied Genetics 118 (2009) 8
- Accession number :
- edsair.doi.dedup.....d912880ab9d9e85dac7f377ef112f4a3