Back to Search
Start Over
Detection of CMV pneumonitis after lung transplantation using PCR of DNA from bronchoalveolar lavage cells
- Source :
- Respiration; international review of thoracic diseases. 67(2)
- Publication Year :
- 2000
-
Abstract
- Background: Cytomegalovirus (CMV) is known as a common pathogen causing infections after lung transplantation. Rapid diagnosis of CMV infection is important for the initiation of a specific treatment. Objective: Evaluation of methods for the rapid diagnosis of CMV pneumonitis. Methods: The detection rates of CMV DNA in bronchoalveolar lavage (BAL) and bronchial brushes by polymerase chain reaction (PCR), of viral antigens (p52 and IE1) in BAL and of pp65 antigen in peripheral blood leukocytes were compared to the clinical status after lung transplantation. In 28 patients, 105 BAL, 96 blood samples and 14 brushes were analyzed. Results: In 6 patients, a total of eight episodes of CMV pneumonitis occurred. Five of the 6 with positive CMV antigens in BAL (p52 or IE1) showed signs of CMV pneumonitis. All episodes of CMV pneumonitis were detected by the PCR of BAL cells. Fourteen samples positive for CMV pp65 antigen in blood were negative in BAL PCR. In these cases, no clinical signs of pulmonary CMV infection occurred. Overall sensitivity, specificity, and positive and negative predictive values for the detection of CMV pneumonitis by PCR of BAL cells were 100, 98.9, 88.9 and 100%, respectively. In brush samples, PCR did not provide additional information to the results of the PCR of BAL cells. Conclusions: PCR of DNA from BAL cells is suitable for reliable and rapid detection of CMV pneumonitis.
- Subjects :
- Pulmonary and Respiratory Medicine
Human cytomegalovirus
Adult
Adolescent
medicine.medical_treatment
Congenital cytomegalovirus infection
Cytomegalovirus
medicine.disease_cause
Bronchoalveolar Lavage
Polymerase Chain Reaction
Sensitivity and Specificity
Herpesviridae
Viral Matrix Proteins
Betaherpesvirinae
Predictive Value of Tests
Leukocytes
Medicine
Lung transplantation
Humans
Prospective Studies
Antigens, Viral
Pneumonitis
biology
medicine.diagnostic_test
business.industry
virus diseases
Pneumonia
respiratory system
medicine.disease
biology.organism_classification
Phosphoproteins
Prognosis
Transplantation
Bronchoalveolar lavage
Immunology
Cytomegalovirus Infections
DNA, Viral
business
Bronchoalveolar Lavage Fluid
Lung Transplantation
Subjects
Details
- ISSN :
- 00257931
- Volume :
- 67
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Respiration; international review of thoracic diseases
- Accession number :
- edsair.doi.dedup.....d90ab128414b3d6112b609e38d81bbe4