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Inhibition by epigallocatechin gallate of CoCl2-induced apoptosis in rat PC12 cells
- Source :
- Life Sciences. 80:1355-1363
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Epigallocatechin-3-gallate (EGCG) is a major constituent of green tea polyphenols. This study was aimed to investigate the possible mechanisms of EGCG-mediated inhibition against apoptosis in rat pheochromocytoma PC12 cells by exposure to CoCl(2). Exposure to CoCl(2) caused the generation of ROS and induced cell death with appearance of apoptotic morphology and DNA fragmentation. However, EGCG rescued the loss of viability in the cells exposed to CoCl(2) and led the reduction of DNA fragmentation and sub-G(1) fraction of cell cycle. Also, EGCG attenuated the CoCl(2)-induced disruption of mitochondrial membrane potential (DeltaPsim), release of cytochrome c from the mitochondria to cytosol and abolished the CoCl(2)-stimulated activities of the caspase cascades, caspase-9 and caspase-3. In addition, EGCG ameliorated the increase in the Bax to Bcl-2 ratio, a marker of apoptosis proceeding, induced by CoCl(2) treatment. Taken together, the present results suggest that EGCG inhibit the CoCl(2)-induced apoptosis of PC12 cells through the mitochondria-mediated apoptosis pathway involved in modulating the Bcl-2 family.
- Subjects :
- Programmed cell death
Blotting, Western
Tetrazolium Salts
Apoptosis
DNA Fragmentation
Epigallocatechin gallate
Mitochondrion
PC12 Cells
complex mixtures
Catechin
General Biochemistry, Genetics and Molecular Biology
Membrane Potentials
chemistry.chemical_compound
Animals
General Pharmacology, Toxicology and Pharmaceutics
Electrophoresis, Agar Gel
chemistry.chemical_classification
Reactive oxygen species
biology
Caspase 3
Cytochrome c
Bcl-2 family
food and beverages
Cell Differentiation
Cobalt
General Medicine
Flow Cytometry
Molecular biology
Caspase 9
Genes, bcl-2
Rats
Thiazoles
Neuroprotective Agents
chemistry
Hypoxia-Ischemia, Brain
Mitochondrial Membranes
biology.protein
DNA fragmentation
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....d8e4f080cbffba22ae4f546139203b75