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Disc Related Cytokines Inhibit Axonal Outgrowth From Dorsal Root Ganglion Cells In Vitro
- Source :
- Spine. 30:621-624
- Publication Year :
- 2005
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2005.
-
Abstract
- Application of nucleus pulposus and disc related cytokines in vitro on cultured dorsal root ganglion (DRG) cells.To study if tumor necrosis factor (TNF) and interleukin-1beta (IL-1beta) may induce similar inhibition of axonal outgrowth from cultured DRG cells as application of nucleus pulposus and to compare a new assessment method to previous data.Pro-inflammatory cytokines related to the intervertebral disc have been suggested to affect adversely neurons following local application, with implications for the nucleus pulposus-induced nerve injury seen in various studies. Nucleus pulposus is known to inhibit axonal outgrowth from cultured DRG cells, thereby indicating a neurotoxic potential. The mechanisms were not understood, but it was suspected that the effect was mediated by pro-inflammatory cytokines produced by the nucleus pulposus.DRG were harvested from newborn rats and put in culture. The axonal outgrowth was determined 24 hours after starting the culture. Twenty-four hours after exposing the cultured cells to nucleus pulposus, frozen nucleus pulposus, TNF, or IL-1beta, the axonal outgrowth was reassessed, and the outgrowth during the exposure time was calculated.Nucleus pulposus clearly reduced the axonal outgrowth. Also, application of TNF and IL-1beta reduced the outgrowth but not as pronounced as the nucleus pulposus. Frozen nucleus pulposus had no effects on the outgrowth. Overall, the data were similar regarding frozen and nonfrozen nucleus pulposus compared to a previous study.It was evident that the 2 studied cytokines inhibited the outgrowth of axons from cultured DRG cells, thus suggesting a neurotoxic potential. However, the inhibition was not as pronounced as for nucleus pulposus. These data may increase our understanding for cytokine induced nerve injury, with implications for future treatment strategies for such conditions.
- Subjects :
- Pathology
medicine.medical_specialty
medicine.medical_treatment
Central nervous system
Rats, Sprague-Dawley
Central nervous system disease
Dorsal root ganglion
Ganglia, Spinal
medicine
Animals
Orthopedics and Sports Medicine
Intervertebral Disc
Cells, Cultured
Cryopreservation
Tumor Necrosis Factor-alpha
business.industry
Anatomy
medicine.disease
Spinal cord
Axons
Rats
Cytokine
medicine.anatomical_structure
Animals, Newborn
nervous system
Cell culture
Tumor necrosis factor alpha
Neurology (clinical)
business
Nucleus
Interleukin-1
Subjects
Details
- ISSN :
- 03622436
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Spine
- Accession number :
- edsair.doi.dedup.....d8e127ed2d76838adc6b1de2bcd80c7e
- Full Text :
- https://doi.org/10.1097/01.brs.0000155410.48700.9e