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Detection of Giardia intestinalis in water samples collected from natural water reservoirs and wells in northern and north-eastern Poland using LAMP, real-time PCR and nested PCR
- Source :
- Journal of water and health. 15(5)
- Publication Year :
- 2017
-
Abstract
- Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal–oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (real-time PCR) and nested PCR. Of the samples examined, Giardia DNA was found in 15 (42%) samples with the use of LAMP; in 12 (33%) of these samples, Giardia DNA from this parasite was also detected using real-time PCR; and in 9 (25%) using nested PCR. Sequencing of selected positive samples confirmed that the PCR products were fragments of the Giardia intestinalis small subunit rRNA gene. Genotyping using multiplex real-time PCR indicated the presence of assemblages A and B, with the latter predominating. The results indicate that surface water in Poland, as well as water taken from surface wells, may be a source of Giardia strains which are potentially pathogenic for humans. It was also demonstrated that LAMP assay is more sensitive than the other two molecular assays.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Genotype
Water Wells
030106 microbiology
Loop-mediated isothermal amplification
Fresh Water
010501 environmental sciences
Biology
Real-Time Polymerase Chain Reaction
01 natural sciences
Polymerase Chain Reaction
Microbiology
law.invention
03 medical and health sciences
law
Parasite hosting
Waste Management and Disposal
Genotyping
Polymerase chain reaction
0105 earth and related environmental sciences
Water Science and Technology
Drinking Water
Public Health, Environmental and Occupational Health
Giardia
Nucleic acid amplification technique
biology.organism_classification
Infectious Diseases
Real-time polymerase chain reaction
Poland
Giardia lamblia
Nested polymerase chain reaction
Nucleic Acid Amplification Techniques
Subjects
Details
- ISSN :
- 14778920
- Volume :
- 15
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of water and health
- Accession number :
- edsair.doi.dedup.....d8d356ba629c154cf646c83ab1e04fc6