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Morphological and enzymatic heterogeneity of suramin-induced lysosomal storage disease in some tissues of mice and rats
- Source :
- Experimental and molecular pathology. 45(1)
- Publication Year :
- 1986
-
Abstract
- Suramin-induced lysosomal storage disease reproduced in the rat was extended to the mouse with the attempt to characterize enzymatically and morphologically heterogeneous responses of various organs to the drug. Suramin administration strikingly decreased (3–6 days afterward) the activity of β-glucuronidase in all tissues studied (kidney, liver, heart, and skeletal muscle). The enzymatic responses were small in the activities of β-N-acetyl-glucosaminidase. The activity of arylsulfatase A decreased to a varying degree in mouse tissues, but in rats the activity increased in liver and skeletal muscle. The activity of cathepsin D increased in rat tissues. Suramin induced morphological changes characteristic to lysosomal storage diseases in kidney and liver but not in heart and skeletal muscle of both mice and rats. Kidney was appreciably more susceptible to suramin than liver. The occurrence of lysosomal accumulations, membranous lamellar inclusions, and granular material were most prominent in tubular cells of kidney and in Kupffer cells of liver. These cells also presented intensive Alcian blue staining. Interestingly, the enzymatic and morphological responses did not corelate with each other, which may reflect differences in the regulation of lysosomal functions in various cell types.
- Subjects :
- Male
medicine.medical_specialty
Cell type
Time Factors
Suramin
Mucopolysaccharidosis
Clinical Biochemistry
Cathepsin D
Biology
Kidney
Pathology and Forensic Medicine
Mice
Internal medicine
Lysosome
Acetylglucosaminidase
medicine
Lysosomal storage disease
Animals
Molecular Biology
Cerebroside-Sulfatase
Glucuronidase
Muscles
Myocardium
Skeletal muscle
medicine.disease
Rats
Disease Models, Animal
Microscopy, Electron
Endocrinology
medicine.anatomical_structure
Liver
Lysosomes
medicine.drug
Carbohydrate Metabolism, Inborn Errors
Subjects
Details
- ISSN :
- 00144800
- Volume :
- 45
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental and molecular pathology
- Accession number :
- edsair.doi.dedup.....d8c9f6b3191baca8dffe5012396a79a3