Hormonal Regulation of α-Lactalbumin Secretion from Bovine Mammary Tissue Cultured in Vitro*

Hormonal control of α-lactalbumin secretion was studied in mammary tissue obtained from nonlactating multiparous Holstein cows pregnant approximately 8 months. Mammary explants were incubated for 72 h in medium containing insulin, T3 and various combinations or concentrations of PRL, cortisol, progesterone, 17β-estradiol, and GH. Concentrations ofα-lactalbumin in the medium and in homogenates of tissue were measured by RIA or sodium dodecyl sulfate-gel electrophoresis as an index of lactogenesis. Increasing quantities of PRL (25 to 100 ng/ml) progressively increased the release of α-lactalbumin into the medium, whereas concentrations of α-lactalbumin in explants exposed to PRL were similar to those in controls. Mammary explants exposed for 3 h to [3H]leucine plus PRL incorporated approximately 2, 11, and 18 times more label into α-lactalbumin 21–24, 45–48, and 69–72 h after initiation of culture, respectively, than explants cultured in the absence of PRL. Cortisol alone had no effect on α-lactalbumin secr...