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Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential
- Source :
- World Journal of Microbiology and Biotechnology, World Journal of Microbiology and Biotechnology, Springer Verlag, 2019, 35 (3), ⟨10.1007/s11274-019-2627-8⟩
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- A halophilic Gram-negative eubacterium was isolated from the Iroise Sea and identified as an efficient producer of polyhydroxyalkanoates (PHA). The strain, designated SF2003, was found to belong to the Halomonas genus on the basis of 16S rRNA gene sequence similarity. Previous biochemical tests indicated that the Halomonas sp. strain SF2003 is capable of supporting various culture conditions which sometimes can be constraining for marine strains. This versatility could be of great interest for biotechnological applications. Therefore, a complete bacterial genome sequencing and de novo assembly were performed using a PacBio RSII sequencer and Hierarchical Genome Assembly Process software in order to predict Halomonas sp. SF2003 metabolisms, and to identify genes involved in PHA production and stress tolerance. This study demonstrates the complete genome sequence of Halomonas sp. SF2003 which contains a circular 4,36 Mbp chromosome, and replaces the strain in a phylogenetic tree. Genes related to PHA metabolism, carbohydrate metabolism, fatty acid metabolism and stress tolerance were identified and a comparison was made with metabolisms of relative species. Genes annotation highlighted the presence of typical genes involved in PHA biosynthesis such as phaA, phaB and phaC and enabled a preliminary analysis of their organization and characteristics. Several genes of carbohydrates and fatty acid metabolisms were also identified which provided helpful insights into both a better knowledge of the intricacies of PHA biosynthetic pathways and of production purposes. Results show the strong versatility of Halomonas sp. SF2003 to adapt to various temperatures and salinity which can subsequently be exploited for industrial applications such as PHA production.
- Subjects :
- DNA, Bacterial
0106 biological sciences
Salinity
Physiology
Sequence assembly
Bacterial genome size
Biology
01 natural sciences
Applied Microbiology and Biotechnology
DNA sequencing
03 medical and health sciences
Genome Size
Stress, Physiological
RNA, Ribosomal, 16S
010608 biotechnology
14. Life underwater
Gene
Phylogeny
ComputingMilieux_MISCELLANEOUS
Genetics
Whole genome sequencing
Base Composition
0303 health sciences
Halomonas
Whole Genome Sequencing
030306 microbiology
Polyhydroxyalkanoates
Fatty Acids
Salt Tolerance
General Medicine
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Halophile
Genes, Bacterial
Carbohydrate Metabolism
Metabolic Networks and Pathways
Bacteria
Biotechnology
Subjects
Details
- ISSN :
- 15730972 and 09593993
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- World Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....d8b7dc925f5eb89ce6cc1e7d11dbc848