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Effects of glyceollin I on vascular contraction in rat aorta
- Source :
- Naunyn-Schmiedeberg's Archives of Pharmacology. 381:517-528
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- The present study was undertaken to investigate the molecular mechanisms by which glyceollin I inhibits vascular contraction in rat aorta. Rat aortic rings were treated with either glyceollin I or vehicle when vascular contraction reached plateaus. We measured the activity of GTP-RhoA and Rho GTPase-activating protein (RhoGAP) and the phosphorylation level of the myosin light chain (MLC(20)), myosin phosphatase targeting subunit 1 (MYPT1), and PKC-potentiated inhibitory protein for heterotrimeric MLCP of 17 kDa (CPI17). Glyceollin I reduced vascular contraction whether endothelium is present or denuded. Glyceollin I reduced vascular contraction induced by NaF, U46619, phenylephrine, or PDBu. Blockers of K(+) channels did not affect the vasorelaxation induced by glyceollin I. Glyceollin I reduced activation of RhoA as well as phosphorylation level of MLC(20). Glyceollin I also reduced phosphorylation of MYPT1 and CPI17 induced by NaF but not PDBu. However, glyceollin I had no direct effect on RhoGAP activation in vitro. Glyceollin I reduced vascular contraction, at least in part, through inhibition of the RhoA/Rho-kinase signaling pathway.
- Subjects :
- Male
medicine.medical_specialty
Myosin Light Chains
Myosin light-chain kinase
RHOA
Pterocarpans
Endothelium
Aorta, Thoracic
In Vitro Techniques
Rats, Sprague-Dawley
chemistry.chemical_compound
Internal medicine
Glyceollin I
medicine
Animals
Phosphorylation
Protein Kinase Inhibitors
Rho-associated protein kinase
Phenylephrine
Glyceollin
Pharmacology
rho-Associated Kinases
biology
General Medicine
Rats
Cell biology
Endocrinology
medicine.anatomical_structure
chemistry
Vasoconstriction
biology.protein
rhoA GTP-Binding Protein
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 14321912 and 00281298
- Volume :
- 381
- Database :
- OpenAIRE
- Journal :
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Accession number :
- edsair.doi.dedup.....d8b6202abd25200f1bd9821fcef8f668