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Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury
- Source :
- Acta Biochimica et Biophysica Sinica. 50:807-816
- Publication Year :
- 2018
- Publisher :
- China Science Publishing & Media Ltd., 2018.
-
Abstract
- Renal ischemia/reperfusion (I/R) is a common cause of acute renal failure in many clinical settings. Our study aimed to elucidate the role of lentiviral vector-mediated KSR1 gene silencing in inflammatory factor expression and proliferation of renal tubular epithelial cells (RTECs) in a rat model of I/R injury. Male Sprague-Dawley (SD) rats were used for I/R model establishment and subject to different treatments, followed by the measurement of neurological severity score (NSS), tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β, 47-kDa heat-shock protein (HSP47), KSR1, and factors related to the Ras/MAPK pathway, as well as cell apoptosis. As compared with the blank group, the neurologic impairment induced by I/R in the siKSR1, U0126, and siKSR1 + U0126 groups was alleviated. Compared with the control group, the other five groups showed increased levels of TNF-α, IL-6, IL-1β, HSP47, N-ras, Raf-1, c-fos, TNF-α, IL-6, p38 MAPK, and cell apoptosis, accompanied by a declined mRNA and protein level of Bcl-2. As compared with the blank and NC groups, the siKSR1, U0126, and siKSR1 + U0126 groups showed decreased levels of TNF-α, IL-6, IL-1β, HSP47, N-ras, Raf-1, c-fos, TNF-α, IL-6, p38 MAPK, cleaved caspase-3, cleaved caspase-9, p53, and cell apoptosis, accompanied by an increased mRNA and protein level of Bcl-2. Our findings demonstrated that KSR1 gene silencing might inhibit the expression of inflammatory factors in RTECs and promote their proliferation by inactivating the Ras/MAPK pathway in the rat model of I/R injury.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
p38 mitogen-activated protein kinases
Genetic Vectors
Biophysics
p38 Mitogen-Activated Protein Kinases
Biochemistry
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Animals
Humans
Gene silencing
Medicine
Gene Silencing
Cell Proliferation
Kidney
Renal ischemia
business.industry
Lentivirus
Epithelial Cells
General Medicine
medicine.disease
Disease Models, Animal
Kidney Tubules
030104 developmental biology
medicine.anatomical_structure
Apoptosis
Reperfusion Injury
030220 oncology & carcinogenesis
Cancer research
Cytokines
Tumor necrosis factor alpha
Inflammation Mediators
business
Protein Kinases
Reperfusion injury
Subjects
Details
- ISSN :
- 16729145
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Acta Biochimica et Biophysica Sinica
- Accession number :
- edsair.doi.dedup.....d8b0f98293a45687db06e8b5cba66a6b