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Accelerated Repair and Reduced Mutagenicity of DNA Damage Induced by Cigarette Smoke in Human Bronchial Cells Transfected with E.coli Formamidopyrimidine DNA Glycosylase
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 1, p e87984 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Cigarette smoke (CS) is associated to a number of pathologies including lung cancer. Its mutagenic and carcinogenic effects are partially linked to the presence of reactive oxygen species and polycyclic aromatic hydrocarbons (PAH) inducing DNA damage. The bacterial DNA repair enzyme formamidopyrimidine DNA glycosylase (FPG) repairs both oxidized bases and different types of bulky DNA adducts. We investigated in vitro whether FPG expression may enhance DNA repair of CS-damaged DNA and counteract the mutagenic effects of CS in human lung cells. NCI-H727 non small cell lung carcinoma cells were transfected with a plasmid vector expressing FPG fused to the Enhanced Green Fluorescent Protein (EGFP). Cells expressing the fusion protein EGFP-FPG displayed accelerated repair of adducts and DNA breaks induced by CS condensate. The mutant frequencies induced by low concentrations of CS condensate to the Na(+)K(+)-ATPase locus (oua(r)) were significantly reduced in cells expressing EGFP-FPG. Hence, expression of the bacterial DNA repair protein FPG stably protects human lung cells from the mutagenic effects of CS by improving cells' capacity to repair damaged DNA.
- Subjects :
- DNA Repair
Pulmonology
DNA repair
DNA damage
Science
Bronchi
Biology
Transfection
Biochemistry
Lung and Intrathoracic Tumors
law.invention
DNA-formamidopyrimidine glycosylase
chemistry.chemical_compound
Molecular cell biology
Genetic Mutation
law
Cell Line, Tumor
Escherichia coli
Genetics
Humans
Multidisciplinary
Escherichia coli Proteins
Cancers and Neoplasms
Smoking Related Disorders
DNA
Formamidopyrimidine DNA glycosylase
Middle Aged
DNA repair protein XRCC4
Molecular biology
Recombinant Proteins
Non-Small Cell Lung Cancer
Nucleic acids
DNA-Formamidopyrimidine Glycosylase
Oncology
chemistry
Recombinant DNA
Medicine
Female
Tobacco Smoke Pollution
DNA Damage
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d8ae9dd8a68c8be938913a56ad236730