Back to Search
Start Over
Diabetes diminishes a typical metabolite of litchi pericarp oligomeric procyanidins (LPOPC) in urine mediated by imbalanced gut microbiota
- Source :
- Food & Function. 12:5375-5386
- Publication Year :
- 2021
- Publisher :
- Royal Society of Chemistry (RSC), 2021.
-
Abstract
- Animal studies and clinical trials have shown that dietary polyphenols and polyphenol-rich foods can reduce the risk of type 2 diabetes (T2D) and its complications, but how diabetes regulates the metabolism of polyphenol has not been fully elucidated. This study investigated the effects of diabetes on litchi pericarp oligomeric procyanidin (LPOPC) dynamic metabolism and its major static metabolites in urine. First, a high-fat and streptozotocin (STZ)-induced diabetic Sprague Dawley (SD) rat model was established. In the diabetic rat model, elevated fasting blood glucose, severely impaired glucose tolerance test, and increased reactive oxygen species (ROS) levels in serum and the liver were observed. Subsequently, 200 mg per kg body weight of LPOPC was administrated to control and diabetic SD rats, and the gastrointestinal tract was collected at 0.5 h, 1 h, 3 h, and 6 h. The results showed that the retention time of LPOPC was not changed in our diabetic rat model. However, the gut microbiota were significantly altered, with elevated Proteobacteria and Verrucomicrobia abundance in diabetic rats and decreased short chain fatty acid (SCFA)-producing bacteria. Interestingly, after one dose of 300 mg per kg body weight LPOPC, the total antioxidant capacity of urine in diabetic rats significantly decreased. We then tested the static metabolites of LPOPC, demonstrating that epicatechin had not changed in urine in diabetic rats, but that shikimic acid was significantly reduced in urine in diabetic rats. The changes in shikimic acid may be due to the alteration of gut microbiota and elevated ROS levels in serum.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Metabolite
Urine
Type 2 diabetes
Gut flora
Antioxidants
Catechin
Diabetes Mellitus, Experimental
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Litchi
RNA, Ribosomal, 16S
Diabetes mellitus
Internal medicine
medicine
Animals
Biflavonoids
Proanthocyanidins
030109 nutrition & dietetics
biology
Plant Extracts
Short-chain fatty acid
Fasting
General Medicine
Metabolism
medicine.disease
biology.organism_classification
Streptozotocin
Gastrointestinal Microbiome
Rats
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
chemistry
Fruit
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 2042650X and 20426496
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Food & Function
- Accession number :
- edsair.doi.dedup.....d8a838247c7033bb2190a4c80b4cf1b8