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Use of electroporation as an option to transform the horn fly, Haematobia irritans : a species recalcitrant to microinjection
- Source :
- Insect Science. 23:621-629
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- The horn fly, Haematobia irritans, is a serious pest of cattle in North America. The control of horn flies has primarily relied on insecticides. However, the heavy use of insecticides has led to the development of insecticide resistance in horn flies. Novel methods to control horn flies are greatly needed. Transgenic technology is an effective tool to genetically modify insects and may lead to novel methods of pest control based on genomic approaches. Here we report a piggyBac-mediated transformation of the horn fly via electroporation. Transformation with a DsRed fluorescent marker protein coding region was verified by PCR analysis of individual fly bodies and pupal cases and sequencing of PCR products. However, Southern blot analysis failed to indicate the DsRed gene was integrated into the horn fly genome. Thus, the electroporation protocol may have caused the DsRed gene to be integrated into bacterial symbionts of the horn fly.
- Subjects :
- 0301 basic medicine
Embryo, Nonmammalian
Microinjections
Sodium Hypochlorite
Genetic Vectors
Genome, Insect
Green Fluorescent Proteins
Genome
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Transformation, Genetic
Animals
Gene
Ecology, Evolution, Behavior and Systematics
Organisms, Genetically Modified
biology
business.industry
Horn (anatomy)
Electroporation
Muscidae
fungi
Pest control
biology.organism_classification
Biotechnology
Cell biology
Haematobia irritans
Transformation (genetics)
030104 developmental biology
Insect Science
DNA Transposable Elements
Cattle
PEST analysis
business
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 17447917 and 16729609
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Insect Science
- Accession number :
- edsair.doi.dedup.....d89e26360f5282f1ccf4157c0ee89689