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Gene Expression Profiling and Secretome Analysis Differentiate Adult-Derived Human Liver Stem/Progenitor Cells and Human Hepatic Stellate Cells
- Source :
- PLoS ONE, PLoS One, Vol. 9, no. 1, p. e86137 [1-11] (2014), PLoS ONE, Vol 9, Iss 1, p e86137 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Adult-derived human liver stem/progenitor cells (ADHLSC) are obtained after primary culture of the liver parenchymal fraction. The cells are of fibroblastic morphology and exhibit a hepato-mesenchymal phenotype. Hepatic stellate cells (HSC) derived from the liver non-parenchymal fraction, present a comparable morphology as ADHLSC. Because both ADHLSC and HSC are described as liver stem/progenitor cells, we strived to extensively compare both cell populations at different levels and to propose tools demonstrating their singularity. ADHLSC and HSC were isolated from the liver of four different donors, expanded in vitro and followed from passage 5 until passage 11. Cell characterization was performed using immunocytochemistry, western blotting, flow cytometry, and gene microarray analyses. The secretion profile of the cells was evaluated using Elisa and multiplex Luminex assays. Both cell types expressed α-smooth muscle actin, vimentin, fibronectin, CD73 and CD90 in accordance with their mesenchymal origin. Microarray analysis revealed significant differences in gene expression profiles. HSC present high expression levels of neuronal markers as well as cytokeratins. Such differences were confirmed using immunocytochemistry and western blotting assays. Furthermore, both cell types displayed distinct secretion profiles as ADHLSC highly secreted cytokines of therapeutic and immuno-modulatory importance, like HGF, interferon-γ and IL-10. Our study demonstrates that ADHLSC and HSC are distinct liver fibroblastic cell populations exhibiting significant different expression and secretion profiles.
- Subjects :
- Male
Proteome
Liver cytology
Microarrays
lcsh:Medicine
Gene Expression
Biology
Biochemistry
Molecular Genetics
Antigens, CD
Molecular Cell Biology
Genetics
Hepatic Stellate Cells
Humans
CD90
Progenitor cell
lcsh:Science
Cells, Cultured
Multidisciplinary
Stem Cells
Gene Expression Profiling
lcsh:R
Mesenchymal stem cell
Infant, Newborn
Computational Biology
Flow Cytometry
Molecular biology
Gene expression profiling
Adult Stem Cells
Liver
Child, Preschool
Hepatic stellate cell
Cytochemistry
Cytokines
lcsh:Q
Female
Stem cell
Cellular Types
Transcriptome
Immunocytochemistry
Cytometry
Adult stem cell
Research Article
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d881b8a3409b2fee2653fb94cef270f1