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Epithelial integrity is maintained by a matriptase-dependent proteolytic pathway
- Source :
- The American journal of pathology. 175(4)
- Publication Year :
- 2009
-
Abstract
- A pericellular proteolytic pathway initiated by the transmembrane serine protease matriptase plays a critical role in the terminal differentiation of epidermal tissues. Matriptase is constitutively expressed in multiple other epithelia, suggesting a putative role of this membrane serine protease in general epithelial homeostasis. Here we generated mice with conditional deletion of the St14 gene, encoding matriptase, and show that matriptase indeed is essential for the maintenance of multiple types of epithelia in the mouse. Thus, embryonic or postnatal ablation of St14 in epithelial tissues of diverse origin and function caused severe organ dysfunction, which was often associated with increased permeability, loss of tight junction function, mislocation of tight junction-associated proteins, and generalized epithelial demise. The study reveals that the homeostasis of multiple simple and stratified epithelia is matriptase-dependent, and provides an important animal model for the exploration of this membrane serine protease in a range of physiological and pathological processes.
- Subjects :
- Aging
Proteolysis
Megacolon
ST14
Epithelium
Permeability
Salivary Glands
Pathology and Forensic Medicine
Tight Junctions
Mice
medicine
Animals
Homeostasis
Matriptase
Alleles
Serine protease
biology
Tight junction
medicine.diagnostic_test
Serine Endopeptidases
Membrane Proteins
Embryo, Mammalian
Transmembrane protein
Cell biology
Intestines
Tamoxifen
medicine.anatomical_structure
Membrane protein
Organ Specificity
biology.protein
Protein Processing, Post-Translational
Gene Deletion
Regular Articles
Subjects
Details
- ISSN :
- 15252191
- Volume :
- 175
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The American journal of pathology
- Accession number :
- edsair.doi.dedup.....d86c7dce0ac14c4e6d7ad52d358e10fa