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Light-induced protein degradation in human-derived cells
- Source :
- Biochemical and Biophysical Research Communications. 487:241-246
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Controlling protein degradation can be a valuable tool for posttranslational regulation of protein abundance to study complex biological systems. In the present study, we designed a light-switchable degron consisting of a light oxygen voltage (LOV) domain of Avena sativa phototropin 1 (AsLOV2) and a C-terminal degron. Our results showed that the light-switchable degron could be used for rapid and specific induction of protein degradation in HEK293 cells by light in a proteasome-dependent manner. Further studies showed that the light-switchable degron could also be utilized to mediate the degradation of secreted Gaussia princeps luciferase (GLuc), demonstrating the adaptability of the light-switchable degron in different types of protein. We suggest that the light-switchable degron offers a robust tool to control protein levels and may serves as a new and significant method for gene- and cell-based therapies.
- Subjects :
- 0301 basic medicine
Phototropins
Phototropin
Avena
Light
Biophysics
Protein degradation
Radiation Dosage
01 natural sciences
Biochemistry
03 medical and health sciences
Genes, Reporter
Humans
Luciferase
Post-translational regulation
Molecular Biology
Gene
biology
010405 organic chemistry
HEK 293 cells
Dose-Response Relationship, Radiation
Cell Biology
Gaussia princeps
biology.organism_classification
0104 chemical sciences
HEK293 Cells
030104 developmental biology
Luminescent Measurements
Proteolysis
Degron
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 487
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....d868b26672f80969ab14e6424b9f4e87