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Immunochemical studies on a Mycoplasma pneumoniae polysaccharide fraction: cross-reactions with type 23 and 32 antipneumococcal rabbit sera
- Source :
- Infection and Immunity. 20:421-429
- Publication Year :
- 1978
- Publisher :
- American Society for Microbiology, 1978.
-
Abstract
- Lipid-free polysaccharide fraction 2 extracted from Mycoplasma pneumoniae strain FH by Prescott et al. (J. Bacteriol. 91:2117-2115, 1966) was examined for its ability to cross-precipitate antibody from type-specific rabbit antipneumococcal sera types 1 to 34 inclusive. Cross-precipitation in type-specific pneumococcal anti-type 23 and anti-type 32 sera was examined in detail and could be attributed to a rhamnose-galactose-rich component of crude M. pneumoniae polysaccharide fraction 2 recovered from immunoprecipitates formed with anti-type 23 serum. Immunochemically isolated mycoplasma polysaccharide was found to contain glucose, galactose, rhamnose, and mannose in 1:14:5:4 molar proportions. Comparison of the ability of 6-O-alpha-L-rhamnosyl-D-glucose and free L-rhamnose to inhibit precepitation by homologous pneumococcal and heterologous mycoplasma polysaccharide antigens indicates a combining site specificity for anti-type 23 and anti-type 32 antibodies directed largely against the alpha-linked L-rhamnosyl determinants and the occurrence of alpha-L-rhamnosyl units in type 32 and M. pneumoniae polysaccharides. Hapten inhibition of the cross-precipitation of pneumococcal type 23 capsular polysaccharide in anti-type 32 serum helps to establish that cross-reactivity can be attributed to interaction of recurrent, alpha-L-rhamnosyl units of type 23 with anit-alpha-L-rhamnoside combining sites of anti-type 32 antibodies.
- Subjects :
- Immunodiffusion
Mycoplasma pneumoniae
Rhamnose
Immunology
Carbohydrates
Mannose
Cross Reactions
Biology
medicine.disease_cause
Microbiology
chemistry.chemical_compound
Mycoplasma
Antigen
Streptococcus pneumoniae
medicine
Antigens, Bacterial
Polysaccharides, Bacterial
Precipitin
Precipitin Tests
Infectious Diseases
chemistry
Parasitology
Hapten
Research Article
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....d8672102079713027388003dfa5722b8