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PTRF/cavin-1 is essential for multidrug resistance in cancer cells
- Source :
- Journal of proteome research. 12(2)
- Publication Year :
- 2012
-
Abstract
- Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.
- Subjects :
- Quantitative proteomics
Caveolin 1
Breast Neoplasms
Biology
medicine.disease_cause
Caveolae
Biochemistry
Membrane Microdomains
PTRF
Cell Line, Tumor
medicine
Humans
ATP Binding Cassette Transporter, Subfamily B, Member 1
RNA, Small Interfering
Lipid raft
Fluorescent Dyes
Gene knockdown
Antibiotics, Antineoplastic
Cholera toxin
RNA-Binding Proteins
General Chemistry
Phosphate-Binding Proteins
Drug Resistance, Multiple
Cell biology
Multiple drug resistance
Gene Expression Regulation, Neoplastic
Doxorubicin
Drug Resistance, Neoplasm
Gene Knockdown Techniques
Cancer cell
lipids (amino acids, peptides, and proteins)
Female
Carrier Proteins
Cavin
Subjects
Details
- ISSN :
- 15353907
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of proteome research
- Accession number :
- edsair.doi.dedup.....d846a54fe1bdb153b8b28036a3889f12