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Bone marrow stroma cells are susceptible to prion infection
- Source :
- Biochemical and Biophysical Research Communications. 377:957-961
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Abnormal protease-resistant prion protein (PrP-res) is the only surrogate biochemical marker for prion diseases, and a sensitive technique to detect PrP-res in blood or tissues is urgently needed. Primary cultured bone marrow stromal cells (MSCs) expressed PrP and were capable of supporting stable human prion infection. Using a mouse-adapted BSE strain, we demonstrated that PrP-res can be detected in expanded MSCs. We then analyzed the bone marrow cells collected at autopsy from two individuals with sporadic Creutzfeldt-Jakob disease (CJD), and, in both cases, cultured MSCs were positive for PrP-res. These data would suggest that ex vivo MSC expansion accompanied by PrP-res analysis could be a helpful tool in the definitive diagnosis of prion disease at an earlier stage in the disease process than is currently possible, and with considerably less distress to the patient.<br />Biochemical and biophysical research communications, 377(3), pp.957-961; 2008
- Subjects :
- Male
Stromal cell
PrPSc Proteins
animal diseases
Biophysics
Bone Marrow Cells
Autopsy
Disease
Biology
Biochemistry
Creutzfeldt-Jakob Syndrome
BSE
MSC
Prion infection
mental disorders
medicine
Animals
Humans
Rats, Wistar
Prion protein
Molecular Biology
Cells, Cultured
Bone marrow stromal cell
Mesenchymal stem cell
Cell Biology
Rats
nervous system diseases
CJD
medicine.anatomical_structure
Immunology
Disease Susceptibility
Bone marrow
Stromal Cells
Ex vivo
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 377
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....d83b9864bccbe939e1249473c230ac86