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Transient downregulation of Sema3A mRNA in a rat model for temporal lobe epilepsy. A novel molecular event potentially contributing to mossy fiber sprouting
- Source :
- Experimental Neurology, 182(1), 142-50. Academic Press, Experimental Neurology, 182(1), 142-150. Academic Press Inc., Holtmaat, A J G D, Gorter, J A, de Wit, J, Tolner, E A, Spijker, S, Giger, R J, Lopes da Silva, F H & Verhaagen, J 2003, ' Transient downregulation of Sema3A mRNA in a rat model for temporal lobe epilepsy-A novel molecular event potentially contributing to mossy fiber sprouting ', Experimental Neurology, vol. 182, no. 1, pp. 142-150 . https://doi.org/10.1016/S0014-4886(03)00035-9, Experimental Neurology, 182, 142-150. Academic Press Inc.
- Publication Year :
- 2003
-
Abstract
- Mossy fiber sprouting (MFS) in the hippocampal dentate gyrus is thought to play a critical role in the hyperexcitability of the hippocampus in temporal lobe epilepsy patients. The composition of molecular signals that is needed to direct this sprouting response has not yet been elucidated to a great extent. In the present study we investigated the expression profile of Sema3A mRNA and the axonal growth-associated protein GAP-43 mRNA during the process of electrically induced epileptogenesis in rats. Sema3A is an axon guidance molecule with repellent activity on dentate granule cell axons. It is produced by neurons in the entorhinal cortex, which synapse on the dendrites of dentate granule cells. Upregulation of GAP-43 expression in granule cells has often been reported in conjunction with MFS. After induction of status epilepticus, the expression of Sema3A mRNA was temporarily downregulated in the entorhinal cortex concomitantly with an upregulation of GAP-43 mRNA in dentate granule cells. In the following days, robust MFS into the dentate molecular layer was observed. When the induction of status epilepticus was incomplete the two responses appeared to dissociate, i.e., the downregulation of Sema3A mRNA did not occur, while upregulation of GAP-43 mRNA in dentate granule cells was still displayed. However, in these rats no significant MFS was observed. These findings indicate that Sema3A mRNA downregulation is temporarily correlated with MFS, while GAP-43 upregulation per se is not, and suggest that a loss of Sema3A in the molecular layer of the dentate gyrus could facilitate MFS into this area during epilepsy. © 2003 Elsevier Science (USA). All rights reserved.
- Subjects :
- Down-Regulation
Nerve Tissue Proteins
Status epilepticus
Biology
Hippocampal formation
Epileptogenesis
Rats, Sprague-Dawley
GAP-43 Protein
Status Epilepticus
Developmental Neuroscience
Downregulation and upregulation
medicine
Journal Article
Animals
Entorhinal Cortex
RNA, Messenger
In Situ Hybridization
Neurons
Dentate gyrus
Research Support, Non-U.S. Gov't
SEMA3A
Electroencephalography
Semaphorin-3A
Granule cell
Entorhinal cortex
Electric Stimulation
Electrodes, Implanted
Rats
Disease Models, Animal
medicine.anatomical_structure
Neurology
Epilepsy, Temporal Lobe
Gene Expression Regulation
Dentate Gyrus
Mossy Fibers, Hippocampal
medicine.symptom
Carrier Proteins
Neuroscience
Subjects
Details
- ISSN :
- 00144886
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- Experimental Neurology
- Accession number :
- edsair.doi.dedup.....d839e6d8eca744202f094553133c8bf6