Back to Search
Start Over
Carp growth hormone: molecular cloning and sequencing of cDNA
- Source :
- Gene. 77:309-315
- Publication Year :
- 1989
- Publisher :
- Elsevier BV, 1989.
-
Abstract
- cDNA clones of the fish Cyprinus carpio growth hormone (GH) mRNA have been isolated from a cDNA library prepared from carp pituitary gland poly(A) + RNA. The nucleotide sequence of one of the carp GH cDNA clones containing an insert of 1164 nucleotides (nt) was determined. The cDNA sequence was found to encode a polypeptide of 210 amino acids (aa) including a signal peptide of 22 aa and to contain 5′ and 3′ untranslated regions of the mRNA of 36 and 498 nt, respectively. The carp GH presents a 63% amino acid sequence homology with the salmon GH, has structural features common with other GH polypeptides of mammalian or avian origin and contains domains of conserved sequence near the N- and C-terminal regions. Southern blot hybridization of carp genomic DNA with GH cDNA probes shows the presence of at least two GH-coding sequences in the fish genome.
- Subjects :
- Carps
Molecular Sequence Data
Restriction Mapping
Cyprinidae
Biology
Conserved sequence
Salmon
Complementary DNA
Genetics
Animals
Genomic library
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Protein Precursors
Carp
Peptide sequence
Southern blot
Base Sequence
cDNA library
Nucleic acid sequence
DNA
General Medicine
Blotting, Northern
biology.organism_classification
Molecular biology
Blotting, Southern
Genes
Growth Hormone
Nucleic Acid Conformation
sense organs
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....d8167f6772d9fdf3e105d9310c170469