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Effect of Herbal Medicine Keishi-To (TJ-45) and Its Components on Rat Pancreatic Acinar Cell Injuries in vivo and in vitro
- Source :
- Pancreatology. 1:102-109
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- In an attempt to clarify the mechanism of the effect of a herbal medicine, Saiko-keishi-to (TJ-10), which is a combination of Keishi-to (TJ-45) and Sho-saiko-to (TJ-9), we investigated the effects of these two herbal medicines and their components on pancreatic acinar cell injury models in vivo and in vitro.Four-week-old male WBN/Kob rats were fed an MB-3 pellet diet containing herbal medicine (TJ-9, TJ-10 and TJ-45). Expressions of pancreatitis-associated protein (PAP) and manganese superoxide dismutase (Mn-SOD) were analyzed with a reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The herbal medicines and two of their components, Keihi (Cinnamomi cortex) and Shakuyaku (Paeoniae radix alba), were tested in vitro using an arginine-treated rat pancreatic acinar AR4-2J cell injury model. The inducible nitric oxide synthase (iNOS) was assayed in in vitro experiments.TJ-45-treated WBN/Kob rats showed no evidence of pancreatitis whereas there were pathological changes of chronic pancreatitis in TJ-9-treated WBN/Kob rats. PAP was not expressed and Mn-SOD expression was increased in the TJ-10-, and TJ-45-treated rats. The herbal medicines and two components suppressed PAP mRNA expression and enhanced Mn-SOD and iNOS mRNA expression in arginine-treated AR4-2J cells.These results suggest that the herbal medicine TJ-45 is effective for chronic pancreatitis caused by pancreatic ischemia.
- Subjects :
- Male
medicine.medical_specialty
Transcription, Genetic
Arginine
Endocrinology, Diabetes and Metabolism
Nitric Oxide Synthase Type II
Pancreatitis-Associated Proteins
Pharmacology
Gene Expression Regulation, Enzymologic
In vivo
Internal medicine
Acinar cell
medicine
Animals
RNA, Messenger
Pancreas
Hepatology
Superoxide Dismutase
Mechanism (biology)
business.industry
Gastroenterology
Rats, Inbred Strains
Manganese Superoxide Dismutase
In vitro
Rats
Endocrinology
Nitric Oxide Synthase
business
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 14243903
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Pancreatology
- Accession number :
- edsair.doi.dedup.....d7f8f0697df0dff115e0ab390629dfe3